Abstract Hydroxyapatite (HAp), a compound similar in composition to human hard tissues such as bone and teeth, has widespread applications in bulk as well as nanoparticle form in numerous fields due to excellent biocompatibility and stability. The increased use of this nanoparticle thus warrants the study of its toxic potential in in vivo models. In this study, the toxicity induced by HAp NPs administered via the oral route using Drosophila as an in vivo model was investigated. Biochemical assays such as lipid peroxidation and nitro blue tetrazolium assay did not reveal toxicity at lowest (10 μg/ml) as well as highest (1000 μg/ml) doses. The HAp nanoparticle-treated flies and larvae did not exhibit signs of behavioral change as evidenced from the negative geotaxis assay and larvae crawling assay, respectively. Survival assay to assess the chronic toxicity of HAp nanoparticles also did not reveal any signs of toxicity at both lowest and highest doses. This demonstrates that hydroxyapatite nanoparticles do not elicit significant in vivo toxicity. Considering the paucity of studies available to support the non-toxic effect of HAp nanoparticles, further investigations are warranted in Drosophila and other in vivo models. Moreover, such HAp nanoparticles can also serve as reliable carrier for drug delivery system.

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评估羟基磷灰石纳米颗粒 - 在黑腹果蝇中诱导的体内毒性

摘要 羟基磷灰石（HAp）是一种成分与人体骨骼和牙齿等硬组织相似的化合物，由于其优异的生物相容性和稳定性，在众多领域中以散装和纳米颗粒形式得到广泛应用。因此，这种纳米颗粒的使用增加，有必要在体内模型中研究其毒性潜力。在这项研究中，使用果蝇作为体内模型，研究了通过口服途径给药的 HAp NPs 诱导的毒性。生化分析如脂质过氧化和硝基蓝四唑分析未显示最低 (10 μg/ml) 和最高 (1000 μg/ml) 剂量的毒性。HAp 纳米颗粒处理的苍蝇和幼虫没有表现出行为变化的迹象，分别从负趋地性试验和幼虫爬行试验中证明。评估 HAp 纳米颗粒慢性毒性的存活试验也没有显示在最低和最高剂量下的任何毒性迹象。这表明羟基磷灰石纳米颗粒不会引起显着的体内毒性。考虑到支持 HAp 纳米粒子的无毒作用的研究很少，有必要在果蝇和其他体内模型中进行进一步研究。此外，这种HAp纳米颗粒还可以作为药物递送系统的可靠载体。需要在果蝇和其他体内模型中进行进一步研究。此外，这种HAp纳米颗粒还可以作为药物递送系统的可靠载体。需要在果蝇和其他体内模型中进行进一步研究。此外，这种HAp纳米颗粒还可以作为药物递送系统的可靠载体。