Glycosylation and phosphorylation are two important mechanisms in organism, both of which can finely regulate protein function respectively or simultaneously. Functional nanoprobes with various reversible interactions of aiming at glycan or phosphate group have been advanced dramatically, however, the comprehensive analysis towards them is largely restricted by the challenge to capture glycopeptides and phosphopeptides at the same time from complex samples due to the limitation of nanoprobe functionalization. Here, we fabricate a promising nanoprobe using the hydrophilic l-cysteine and titanium ions to enhance the phosphopeptides capture, meanwhile, realize the glycopeptides capture. We test the performance of the promising nanoprobe, showing that it is not only equipped with good unbiased ability in glycopeptides capture with high sensitivity and selectivity, but also highly effective for phosphopeptides capture. More importantly, the promising nanoprobe enables the simultaneous capture and analysis of glycosylated and phosphorylated peptides from mouse brain, as well as human eye lens of normal and cataract. The identification of glycosylated and phosphorylated sites within normal and cataract reflects their differential expression and offers an excellent insight to glycosylation/phosphorylation-related human eye diseases, indicating its great potential in glycoproteomics and phosphoproteomics researches.

中文翻译：

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基于亲水相互作用液相色谱和固定化金属亲和色谱的用于捕获糖肽和磷酸肽的有前景的纳米探针

糖基化和磷酸化是生物体中的两种重要机制，两者都可以分别或同时精细调节蛋白质功能。具有针对聚糖或磷酸基团的各种可逆相互作用的功能性纳米探针已取得显着进展，然而，由于纳米探针功能化的限制，对它们的综合分析在很大程度上受到从复杂样品中同时捕获糖肽和磷酸肽的挑战的限制. 在这里，我们使用亲水性 l-半胱氨酸和钛离子制造了一种有前途的纳米探针，以增强磷酸肽的捕获，同时实现糖肽的捕获。我们测试了有前途的纳米探针的性能，表明它不仅在糖肽捕获中具有良好的无偏能力，具有高灵敏度和选择性，而且对磷酸肽捕获也非常有效。更重要的是，有前途的纳米探针能够同时捕获和分析来自小鼠大脑以及正常和白内障的人眼晶状体的糖基化和磷酸化肽。正常和白内障中糖基化和磷酸化位点的鉴定反映了它们的差异表达，并为糖基化/磷酸化相关的人眼疾病提供了极好的见解，表明其在糖蛋白质组学和磷酸蛋白质组学研究中的巨大潜力。有前途的纳米探针能够同时捕获和分析来自小鼠大脑以及正常和白内障的人眼晶状体的糖基化和磷酸化肽。正常和白内障中糖基化和磷酸化位点的鉴定反映了它们的差异表达，并为糖基化/磷酸化相关的人眼疾病提供了极好的见解，表明其在糖蛋白质组学和磷酸蛋白质组学研究中的巨大潜力。有前途的纳米探针能够同时捕获和分析来自小鼠大脑以及正常和白内障的人眼晶状体的糖基化和磷酸化肽。正常和白内障中糖基化和磷酸化位点的鉴定反映了它们的差异表达，并为糖基化/磷酸化相关的人眼疾病提供了极好的见解，表明其在糖蛋白质组学和磷酸蛋白质组学研究中的巨大潜力。