DNA ing（III）：用于大规模时控发光和基于生命的传感的智能“化学鼻子/舌头”,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

DNA ing（III）：用于大规模时控发光和基于生命的传感的智能“化学鼻子/舌头”
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-02-13 00:00:00 , DOI: 10.1021/acs.analchem.7b05167
Shi-Fan Xue ₁ , Zi-Han Chen ₁ , Xin-Yue Han ₁ , Zi-Yang Lin ₁ , Qi-Xian Wang ₁ , Min Zhang ₁ , Guoyue Shi ₁

Affiliation

近年来，基于模式的传感器发展迅速，这是因为它们具有仅用很少的探针即可检测和区分大量分析物的潜力。但是，没有人发现或使用DNA和ter（Tb）的组合作为大规模混合测量方法的模式识别系统。在这里，我们首次报告DNA敏化的Tb（DNA / Tb）作为一种无标签且用途广泛的“化学鼻/舌”，可用于金属离子的大规模时控发光（TGL）监测以具有成本效益的方式涵盖几乎整个元素周期表。筛选了一系列富含鸟嘌呤/胸腺嘧啶（G / T）的DNA配体，以敏化Tb的发光（指天线效应）作为对溶液中金属离子的智能模式响应者，金属离子与DNA的相互作用可以差异地改变DNA对Tb的天线效应，即模式信号。我们的结果表明，只有3个无DNA / Tb标记的传感器可以成功地区分49种分析物，包括碱金属离子，碱土金属离子，过渡/过渡金属离子和镧系元素离子。对49种金属的盲测进一步证实了DNA / Tb传感器的识别能力。此外，还展示了使用DNA / Tb传感器的基于寿命的模式识别应用程序。这种DNA / Tb模式识别策略可以扩展为构建一系列“化学鼻/舌”，以通过使用不同的响应性DNA配体来监测各种生化物种，从而为传感应用和涉及DNA的研究提供了一种多功能而强大的工具分子相互作用。

"点击查看英文标题和摘要"

DNA Encountering Terbium(III): A Smart “Chemical Nose/Tongue” for Large-Scale Time-Gated Luminescent and Lifetime-Based Sensing

Recent years have witnessed the rapid development of pattern-based sensors due to their potential to detect and differentiate a wealth of analytes with only few probes. However, no one has found or used the combination of DNA and terbium(III) (Tb) as a pattern recognition system for large-scale mix-and-measure assays. Here we report for the first time that DNA-sensitized Tb (DNA/Tb), as a label-free and versatile “chemical nose/tongue”, can be employed for wide-scale time-gated luminescent (TGL) monitoring of metal ions covering nearly the entire periodic table in a cost-effective fashion. A series of guanine/thymine (G/T)-rich DNA ligands was screened to sensitize the luminescence of Tb (referring to the antenna effect) as smart pattern responders to metal ions in solution, and metal ion–DNA interactions can differentially alter the antenna effect of DNA toward Tb as pattern signals. Our results show that as few as 3 DNA/Tb label-free sensors could successfully discriminate 49 analytes, including alkali-metal ions, alkaline-earth-metal ions, transition/post-transition metal ions, and lanthanide ions. A blind test with 49 metals further confirmed the discriminating power of DNA/Tb sensors. Moreover, the lifetime-based pattern recognition application using DNA/Tb sensors was also demonstrated. This DNA/Tb pattern recognition strategy could be extended to construct a series of “chemical noses/tongues” for monitoring various biochemical species by using different responsive DNA ligands, thus promising a versatile and powerful tool for a sensing application and investigation of DNA-involving molecular interactions.

更新日期：2018-02-13

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南