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Biosynthesis, structure and antioxidant activities of xanthan gum from Xanthomonas campestris with additional furfural
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2019-04-05 , DOI: 10.1016/j.carbpol.2019.04.018
Yan Kang , Panyu Li , Xiaotong Zeng , Xi Chen , Yi Xie , Yu Zeng , Yongkui Zhang , Tonghui Xie

Lignocellulosic-like materials are potentially low-cost fermentation substrates, but their pretreatment brings about by-products. This work investigated the effects of furfural on xanthan gum (XG) production, and product quality was evaluated by structure, viscosity and antioxidant capacities. Xanthomonas campestris maintained steady polysaccharide yield (above 13 g·L–1) with enhanced cell growth at low furfural concentrations (below 3.2 g·L–1). The products were verified as XG by FT-IR, XRD, NMR and monosaccharide analysis. Moreover, they were found to have reduced acetyl, rising pyruvate and up-to-down glucuronic acid groups as increasing furfural concentration. Furthermore, XG product with 1 g·L–1 furfural addition showed the best hydroxyl scavenging effects, though reducing powers presented no variation. It was demonstrated that furfural, the common hydrolysis by-product, was not necessarily an inhibitor for fermentation, and an appropriate amount of furfural was beneficial to XG production with steady yield and good quality.



中文翻译:

樟脑黄单胞菌与其他糠醛的黄原胶的生物合成,结构和抗氧化活性

木质纤维素类材料可能是低成本的发酵底物,但其预处理会带来副产物。这项工作研究了糠醛对黄原胶(XG)生产的影响,并通过结构,粘度和抗氧化能力评估了产品质量。油菜黄单胞菌Xanthomonas campestris)在低糠醛浓度(低于3.2 g·L –1)下保持稳定的多糖产量(高于13 g·L –1)并具有增强的细胞生长。产物通过FT-IR,XRD,NMR和单糖分析确认为XG。此外,发现随着糠醛浓度的增加,它们的乙酰基减少,丙酮酸增加和葡萄糖醛酸基团的增加到减少。此外,XG产品的含量为1 g·L –1糠醛添加显示出最佳的羟基清除效果,尽管还原能力没有变化。结果表明,糠醛是常见的水解副产物,不一定是发酵的抑制剂,适量的糠醛有利于XG的生产,具有稳定的收率和良好的品质。

更新日期:2019-04-07
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