Pulping wastewater still contains massive refractory organics after biotreatment, with high colority, low biodegradability, and lasting biotoxicity. To eliminate refractory organics in pulping wastewater, a methanotrophic co-metabolic system in a gas cycle Sequencing Batch Biofilm Reactor (gcSBBR) seeded by soil at a ventilation opening of coal mine was quickly built on the 92nd day. The removal rate of COD, colority and TOC was 53.28%, 50.59% and 51.60%, respectively. Analysis of 3D-EEM indicated that glycolated protein-like, melanoidin-like or lignocellulose-like, and humic acid-like decreased by 7.85%, 5.02% and 1.74%, respectively. Moreover, this system exhibited high activity of soluble methane monooxygenase (sMMO) and mmoX encoding sMMO reached up to 7.89 × 10⁵ copies/μL. Methanotrophs, namely, Methylocaldum (8.28%), Methylococcus (6.06%) and Methylomonas (0.07%), were detected by 16S rRNA sequencing. And other bacteria were dominated by Denitratisoma, Anaerolineaceae_uncultured and Methylophilaceae_uncultured. Refractory organics was biodegraded through the synergy among microorganisms, and a postulated synergy pathway was put forward.

制浆废水经过生物处理后仍含有大量难处理的有机物，具有较高的色度，较低的生物降解性和持久的生物毒性。为了消除制浆废水中的难处理有机物，在第92天迅速建立了由土壤播种在煤矿通风口的气体循环定序生物膜反应器（gcSBBR）中的甲烷营养共代谢系统。COD，色度和TOC的去除率分别为53.28％，50.59％和51.60％。对3D-EEM的分析表明，糖基化蛋白样，类黑素蛋白样或木质纤维素样和腐殖酸样分别降低了7.85％，5.02％和1.74％。此外，该系统表现出高活性的可溶性甲烷单加氧酶（sMMO），编码sMMO的mmoX达到7.89×10 ⁵ 拷贝/μL。甲烷营养菌，即通过16S rRNA测序检测到了甲基局部细菌（8.28％），甲基球菌（6.06％）和甲基单胞菌（0.07％）。和其他细菌通过主导Denitratisoma，Anaerolineaceae _uncultured和Methylophilaceae _uncultured。难降解有机物通过微生物之间的协同作用而被生物降解，并提出了一种假定的协同作用途径。