Real-Time in Situ Visualizing of the Sequential Activation of Caspase Cascade Using a Multicolor Gold-Selenium Bonding Fluorescent Nanoprobe.,Analytical Chemistry

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Real-Time in Situ Visualizing of the Sequential Activation of Caspase Cascade Using a Multicolor Gold-Selenium Bonding Fluorescent Nanoprobe.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-04-03 00:00:00 , DOI: 10.1021/acs.analchem.9b00452
Xiaojun Liu ₁ , Xiaoxiao Song ₁ , Dongrui Luan ₁ , Bo Hu ₁ , Kehua Xu ₁ , Bo Tang ₁

Affiliation

The caspase cascade is an ensemble of very important signaling molecules that plays a critical role in cell apoptosis. Real-time monitoring of the upstream and downstream activation relationships of the caspases in the signal pathway is of great significance for understanding the regulatory mechanisms of these signaling molecules in the development of various diseases. Herein, a multicolor fluorescent nanoprobe, GNP-Se-Casp, has been developed based on Au–Se bonding for real-time in situ monitoring caspase- (casp-) 3, 8, and 9 during cell apoptosis. In the real-time fluorescence imaging of apoptotic HeLa cells induced by staurosporine using GNP-Se-Casp, the fluorescence signals corresponding to casp-8 and casp-9 sequentially turn on, followed by the appearance of the fluorescence of casp-3, which visualizes the upstream and downstream relationships of casp-3, -8, and -9. Thus, GNP-Se-Casp is an effective tool for real-time in situ monitoring of caspase cascade activation in the apoptosis process of tumor cells. This design strategy is easily adaptable to in situ detection of other signal molecules, especially those with upstream and downstream activation relationships.

中文翻译：

使用多色金-硒键合荧光纳米探针对Caspase级联序列激活的实时原位可视化。

半胱天冬酶级联反应是非常重要的信号分子的集合，在细胞凋亡中起着至关重要的作用。胱天蛋白酶在信号通路中的上游和下游激活关系的实时监测对于理解这些信号分子在各种疾病发展中的调控机制具有重要意义。在此，基于Au-Se键开发了一种多色荧光纳米探针GNP-Se-Casp，用于在细胞凋亡过程中实时原位监测caspase-（casp-）3、8和9。在使用GNP-Se-Casp对星形孢菌素诱导的凋亡HeLa细胞进行实时荧光成像时，对应于casp-8和casp-9的荧光信号依次打开，然后出现casp-3的荧光，可视化了casp-3，-8和-9的上游和下游关系。因此，GNP-Se-Casp是实时实时监测肿瘤细胞凋亡过程中caspase级联激活的有效工具。这种设计策略很容易适用于其他信号分子的原位检测，尤其是具有上游和下游激活关系的信号分子。

更新日期：2019-04-03

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