在室温下以非常规的梯状排列轻松构建 DNA 四面体,Nanoscale Advances

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在室温下以非常规的梯状排列轻松构建 DNA 四面体
Nanoscale Advances ( IF 4.6 ) Pub Date : 2018-12-27 , DOI: 10.1039/c8na00323h
Ziwen Dai ₁ , Hoi Man Leung ₁ , Qi Gao ₂ , Fei Wang ₁ , Sze Wing Wong ₁ , Ling Sum Liu ₁ , Yu Ju Au ₁ , King Wai Chiu Lai ₂ , Pik Kwan Lo _{1,

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Affiliation

DNA四面体作为最经典、最简单的三维DNA纳米结构已被广泛应用于生物医学和生物传感领域。然而，现有的组装方法通常需要苛刻的热退火条件，涉及形成不需要的副产物，并且尺寸控制不佳。本文报道了一种在室温下以定量产率将离散 DNA 四面体制造为单一、热力学稳定的产物的简便策略。该系统不需要 DNA 触发或热退火处理来启动自组装。这种DNA四面体由三个化学连接的三角形DNA组成，呈非常规的梯状排列，测量高度为~4.16±0.04 nm，显示出对生物环境中酶降解的额外保护。通过温度、能量依赖性和网格蛋白介导的内吞途径。这些特性使我们的 DNA 四面体可用作运输非常小且对温度敏感的货物的工具。这种为 DNA 四面体开发的新型组装策略可能会扩展到其他高度复杂的多面体；这表明了它的普遍性。

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Facile construction of a DNA tetrahedron in unconventional ladder-like arrangements at room temperature

A DNA tetrahedron as the most classical and simplest three-dimensional DNA nanostructure has been widely utilized in biomedicine and biosensing. However, the existing assembly approaches usually require harsh thermal annealing conditions, involve the formation of unwanted by-products, and have poor size control. Herein, a facile strategy to fabricate a discrete DNA tetrahedron as a single, thermodynamically stable product in a quantitative yield at room temperature is reported. This system does not require a DNA trigger or thermal annealing treatment to initiate self-assembly. This DNA tetrahedron was made of three chemically ligated triangular-shaped DNAs in unconventional ladder-like arrangements, with measured heights of ∼4.16 ± 0.04 nm, showing extra protections for enzymatic degradation in biological environment. They show substantial cellular uptake in different cell lines via temperature, energy-dependent and clathrin-mediated endocytosis pathways. These characteristics allow our DNA tetrahedron to be used as vehicles for the delivery of very small and temperature-sensitive cargos. This novel assembly strategy developed for DNA tetrahedra could potentially be extended to other highly complex polyhedra; this indicated its generalizability.

更新日期：2019-04-01

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