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Polypyrrole-coated phase-change liquid perfluorocarbon nanoparticles for the visualized photothermal-chemotherapy of breast cancer.
Acta Biomaterialia ( IF 9.4 ) Pub Date : 2019-03-29 , DOI: 10.1016/j.actbio.2019.03.056 Qiang Yang 1 , Pan Li 2 , Haitao Ran 2 , Jingyuan Wan 1 , Huan Chen 1 , Huali Chen 1 , Zhigang Wang 2 , Liangke Zhang 1
Acta Biomaterialia ( IF 9.4 ) Pub Date : 2019-03-29 , DOI: 10.1016/j.actbio.2019.03.056 Qiang Yang 1 , Pan Li 2 , Haitao Ran 2 , Jingyuan Wan 1 , Huan Chen 1 , Huali Chen 1 , Zhigang Wang 2 , Liangke Zhang 1
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A theranostic nanoplatform (DTX/PFH@PPy-FA) for multi-modal imaging-guided photothermal-chemotherapy has been constructed. Lipid-perfluorohexane (PFH) nanodroplet loaded with docetaxel (DTX) was coated with a polypyrrole (PPy) shell. Then the folic acid (FA) molecule with active tumor-targeting function was modified on the surface of PPy shell. Due to the good photothermal conversion performance, PPy shell can raise the temperature under the near infrared laser irradiation, which not only produces photothermal effect to kill tumor cells, but also promotes liquid-gas phase change of PFH, and produces ultrasound imaging effect. The results of photothermal experiment and imaging experiment confirmed that the obtained DTX/PFH@PPy-FA possessed good photothermal, photoacoustic imaging and ultrasound imaging effects in vitro and in vivo. The results of in vitro cell experiments showed that DTX/PFH@PPy-FA had a active targeting ability to tumor cells, and its photothermal-chemotherapy synergistically inhibited the proliferation of tumor cells. In vivo study on 4T1-bearing BALB/c mice indicated that the photothermal-chemotherapy of DTX/PFH@PPy-FA not only effectively inhibited the growth of 4T1 breast cancer, but also inhibited lung metastasis. This multifunctional nanoparticle is expected to become a new nanoplatform for the visualized photothermal-chemotherapy of cancer. STATEMENT OF SIGNIFICANCE: In this work, we presented a multi-modal imaging-guided photothermal-chemotherapy theranostic nanoplatform (DTX/PFH@PPy-FA) for visualized treatment of breast cancer. The docetaxel (DTX) loaded perfluorohexane (PFH) nanodroplets (DTX/PFH@SPC) were firstly prepared and then coated with polypyrrole shell (PPy). Then, PEGylated folic acid was covalently modified to obtain the folate-targeted multifunctional nanoparticle (DTX/PFH@PPy-FA). Due to the good photothermal conversion performance, PPy shell can raise the temperature under the near infrared laser irradiation, which not only produces photothermal effect to kill tumor cells, but also promotes liquid-gas phase change of PFH, and produces good ultrasound imaging effect. The PPy shell also imparts photoacoustic imaging characteristics to the nanoparticles. Experimental results show that our prepared DTX/PFH@PPy-FA possesses folic acid-mediated tumor targeting ability, ultrasound and photoacoustic imaging, and photothermal-chemotherapy synergistic effect. This multi-functional nanoparticle is expected to become a new platform for the visualized photothermal-chemotherapy of breast cancer.
中文翻译：
聚吡咯涂层相变液体全氟化碳纳米粒子用于可视化乳腺癌的光热化学疗法。
构造了用于多模式成像引导光热化学疗法的治疗学纳米平台（DTX / PFH @ PPy-FA）。装载多西他赛（DTX）的脂质全氟己烷（PFH）纳米滴涂有聚吡咯（PPy）壳。然后在PPy壳的表面修饰具有活性的靶向肿瘤功能的叶酸（FA）分子。由于具有良好的光热转换性能，PPy壳可以在近红外激光照射下升高温度，不仅产生光热效应杀死肿瘤细胞，而且促进PFH的液相-液相变化，并产生超声成像效果。光热实验和成像实验的结果证实，所获得的DTX / PFH @ PPy-FA在体内外均具有良好的光热，光声成像和超声成像效果。体外细胞实验结果表明，DTX / PFH @ PPy-FA具有对肿瘤细胞的主动靶向能力，其光热化学疗法协同抑制肿瘤细胞的增殖。对携带4T1的BALB / c小鼠的体内研究表明，DTX / PFH @ PPy-FA的光热化学疗法不仅有效抑制4T1乳腺癌的生长，而且抑制了肺转移。预期该多功能纳米颗粒将成为可视化癌症光热化学疗法的新纳米平台。重大意义声明：在这项工作中，我们提出了一种多模态成像引导的光热化学疗法治疗法纳米平台（DTX / PFH @ PPy-FA），用于可视化治疗乳腺癌。首先制备载有多西紫杉醇（DTX）的全氟己烷（PFH）纳米液滴（DTX / PFH @ SPC），然后用聚吡咯壳（PPy）涂覆。然后，对聚乙二醇化叶酸进行共价修饰，得到以叶酸为靶标的多功能纳米颗粒（DTX / PFH @ PPy-FA）。由于具有良好的光热转化性能，PPy壳可以在近红外激光照射下升高温度，不仅产生光热效应杀死肿瘤细胞，而且促进了PFH的液相-液相变化，并产生了良好的超声成像效果。PPy壳还赋予纳米颗粒光声成像特性。实验结果表明，我们制备的DTX / PFH @ PPy-FA具有叶酸介导的肿瘤靶向能力，超声和光声成像以及光热化学疗法的协同作用。
更新日期：2019-04-01
中文翻译：
聚吡咯涂层相变液体全氟化碳纳米粒子用于可视化乳腺癌的光热化学疗法。
构造了用于多模式成像引导光热化学疗法的治疗学纳米平台（DTX / PFH @ PPy-FA）。装载多西他赛（DTX）的脂质全氟己烷（PFH）纳米滴涂有聚吡咯（PPy）壳。然后在PPy壳的表面修饰具有活性的靶向肿瘤功能的叶酸（FA）分子。由于具有良好的光热转换性能，PPy壳可以在近红外激光照射下升高温度，不仅产生光热效应杀死肿瘤细胞，而且促进PFH的液相-液相变化，并产生超声成像效果。光热实验和成像实验的结果证实，所获得的DTX / PFH @ PPy-FA在体内外均具有良好的光热，光声成像和超声成像效果。体外细胞实验结果表明，DTX / PFH @ PPy-FA具有对肿瘤细胞的主动靶向能力，其光热化学疗法协同抑制肿瘤细胞的增殖。对携带4T1的BALB / c小鼠的体内研究表明，DTX / PFH @ PPy-FA的光热化学疗法不仅有效抑制4T1乳腺癌的生长，而且抑制了肺转移。预期该多功能纳米颗粒将成为可视化癌症光热化学疗法的新纳米平台。重大意义声明：在这项工作中，我们提出了一种多模态成像引导的光热化学疗法治疗法纳米平台（DTX / PFH @ PPy-FA），用于可视化治疗乳腺癌。首先制备载有多西紫杉醇（DTX）的全氟己烷（PFH）纳米液滴（DTX / PFH @ SPC），然后用聚吡咯壳（PPy）涂覆。然后，对聚乙二醇化叶酸进行共价修饰，得到以叶酸为靶标的多功能纳米颗粒（DTX / PFH @ PPy-FA）。由于具有良好的光热转化性能，PPy壳可以在近红外激光照射下升高温度，不仅产生光热效应杀死肿瘤细胞，而且促进了PFH的液相-液相变化，并产生了良好的超声成像效果。PPy壳还赋予纳米颗粒光声成像特性。实验结果表明，我们制备的DTX / PFH @ PPy-FA具有叶酸介导的肿瘤靶向能力，超声和光声成像以及光热化学疗法的协同作用。
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