The development of microbial electrolysis cells (MECs) for methane production from waste activated sludge (WAS) is arrested due to the limited methane yield and fragile system stability. This study proposed a strategy to accelerate and stabilize MEC via 1.0 g/g DM (dry matter) sludge-based biochar (BC). The results showed that BC clearly accelerated methane production by 24.7% and enhanced VS removal efficiency by 17.9%, compared to control group. Variations of SCOD, proteins, carbohydrates and VFAs indicated biochar promoted hydrolysis and acidogenesis process. Cyclic voltammetry (CV) curves and coulombic efficiency (CE) suggested organic matters degradation and electron generation on anode were enhanced with supplement of biochar. Microbial community analyses revealed that biochar addition could both promote DIET through substituting exoelectrogen (e.g., ) on anode and enrich hydrogenotrophic methanogens (e.g., ) on cathode, which is beneficial to development of MEC as to methane recovery from organic matters.

中文翻译：

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污泥基生物炭辅助微生物电解池中废弃活性污泥高温厌氧消化生产甲烷


由于甲烷产量有限和系统稳定性脆弱，用于从废弃活性污泥（WAS）生产甲烷的微生物电解池（MEC）的发展受到阻碍。本研究提出了一种通过 1.0 g/g DM（干物质）污泥基生物炭 (BC) 加速和稳定 MEC 的策略。结果表明，与对照组相比，BC 明显加快了甲烷产量 24.7%，VS 去除效率提高了 17.9%。 SCOD、蛋白质、碳水化合物和VFA的变化表明生物炭促进了水解和产酸过程。循环伏安（CV）曲线和库仑效率（CE）表明，添加生物炭可以增强有机物的降解和阳极上电子的产生。微生物群落分析表明，生物炭的添加既可以通过替代阳极上的外产电促进DIET，又可以富集阴极上的氢营养产甲烷菌（例如，），有利于MEC从有机物中回收甲烷的发展。