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Absorption Characteristics of Chitobiose and Chitopentaose in the Human Intestinal Cell Line Caco-2 and Everted Gut Sacs
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-03-30 00:00:00 , DOI: 10.1021/acs.jafc.9b01355 Ping Chen , Mengyao Zhao , Qiming Chen , Liqiang Fan , Feng Gao , Liming Zhao 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-03-30 00:00:00 , DOI: 10.1021/acs.jafc.9b01355 Ping Chen , Mengyao Zhao , Qiming Chen , Liqiang Fan , Feng Gao , Liming Zhao 1
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The everted gut sacs and Caco-2 cell models were used to investigate the intestinal absorptive characteristics and subcellular localization of chitobiose and chitopentaose in this study. In everted gut sacs, the absorptive pattern showed no concentration-dependent manner when the concentration was lower than 10 mM. In the presence of phlorizin (100 μM) and phloretin (100 μM), the chitobiose absorption rates decreased by (4.97 ± 0.89)% and (19.2 ± 2.77)%, and they were (10.4 ± 2.43)% and (27.5 ± 1.68)% for chitopentaose. In Caco-2 cells, the concentration showed influences similar to those with the everted gut sacs results. After adding phlorizin and phloretin in the apical side, the PappAP–BL of chitobiose and chitopentaose significantly decreased. Considering the translocation, they were enriched in endoplasmic reticulum and mitochondrion. This study indicated that concentration and active transporter were capable of mediating the absorption of chitobiose and chitopentaose, and the subcellular localization of them could help to study the mechanisms of their effects.
中文翻译：
壳聚糖和壳聚糖在人肠道细胞系Caco-2和肠外翻囊囊中的吸收特性
在本研究中，使用外翻的肠囊和Caco-2细胞模型研究了壳二糖和几丁质糖的肠道吸收特性和亚细胞定位。在肠外翻囊中，当浓度低于10 mM时，吸收模式没有浓度依赖性。在Phlorizin（100μM）和Phlooretin（100μM）存在下，壳二糖吸收率分别降低了（4.97±0.89）％和（19.2±2.77）％，分别为（10.4±2.43）％和（27.5±1.68） ）％的几丁质糖。在Caco-2细胞中，浓度显示出与肠外翻囊结果相似的影响。在顶侧添加了根皮苷和根皮素后，P appAP–BL壳二糖和壳五糖的含量显着降低。考虑到易位，它们富含内质网和线粒体。这项研究表明，浓度和活性转运蛋白能够介导壳二糖和壳多糖的吸收，并且它们在亚细胞中的定位可以帮助研究它们的作用机理。
更新日期：2019-03-30
中文翻译：
壳聚糖和壳聚糖在人肠道细胞系Caco-2和肠外翻囊囊中的吸收特性
在本研究中，使用外翻的肠囊和Caco-2细胞模型研究了壳二糖和几丁质糖的肠道吸收特性和亚细胞定位。在肠外翻囊中，当浓度低于10 mM时，吸收模式没有浓度依赖性。在Phlorizin（100μM）和Phlooretin（100μM）存在下，壳二糖吸收率分别降低了（4.97±0.89）％和（19.2±2.77）％，分别为（10.4±2.43）％和（27.5±1.68） ）％的几丁质糖。在Caco-2细胞中，浓度显示出与肠外翻囊结果相似的影响。在顶侧添加了根皮苷和根皮素后，P appAP–BL壳二糖和壳五糖的含量显着降低。考虑到易位，它们富含内质网和线粒体。这项研究表明，浓度和活性转运蛋白能够介导壳二糖和壳多糖的吸收，并且它们在亚细胞中的定位可以帮助研究它们的作用机理。
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