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Changes in Nanoscale Chain Assembly in Sweet Potato Starch Lamellae by Downregulation of Biosynthesis Enzymes
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-03-29 00:00:00 , DOI: 10.1021/acs.jafc.8b06523 Binjia Zhang 1 , Wenzhi Zhou 2 , Dongling Qiao 3 , Peng Zhang 2 , Siming Zhao 1 , Liang Zhang 4 , Fengwei Xie 5, 6, 7
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-03-29 00:00:00 , DOI: 10.1021/acs.jafc.8b06523 Binjia Zhang 1 , Wenzhi Zhou 2 , Dongling Qiao 3 , Peng Zhang 2 , Siming Zhao 1 , Liang Zhang 4 , Fengwei Xie 5, 6, 7
Affiliation
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Granule-bound starch synthase I (GBSSI) and starch-branching enzymes I and II (SBEI and SBEII) are crucial enzymes that biosynthesize starches with varied apparent amylose contents and amylopectin branching structure. With a sweet potato (Ipomoea batatas [L.] Lam. cv. Xushu22), this work shows that downregulating GBSSI (for waxy starch) or SBE (for high-amylose starch) activity allowed the formation of new semicrystalline lamellae (named Type II) in sweet potato starch in addition to the widely reported Type I lamellae. Small-angle X-ray scattering (SAXS) results show that, compared with Type I lamellae, Type II lamellae displayed increased average thickness and thickness-distribution width, with thickened amorphous and crystalline components. The size-exclusion-chromatography (SEC) data revealed mainly two enzyme sets, (i) and (ii), synthesizing amylopectin chains. Reducing the GBSSI or SBE activity increased the amounts of amylopectin long chains (degree of polymerization (DP) ≥ 33). Combined SAXS and SEC analyses indicate that parts of these long chains from enzyme set (i) could be confined to Type II lamellae, followed by DP ≤ 32 short chains in Type I lamellae and the rest of the long chains from enzyme sets (i) and (ii) spanning more than a single lamella.
中文翻译：
下调生物合成酶的甘薯淀粉薄片中纳米链组装的变化。
颗粒结合的淀粉合酶I（GBSSI）和淀粉分支酶I和II（SBEI和SBEII）是至关重要的酶，可以生物合成具有不同表观直链淀粉含量和支链淀粉分支结构的淀粉。搭配红薯（番薯）[L.]林。简历。Xushu22），这项工作表明，下调GBSSI（对于蜡状淀粉）或SBE（对于高直链淀粉）活性，除了广泛报道的I型薄片之外，还可以在甘薯淀粉中形成新的半结晶薄片（命名为II型）。小角X射线散射（SAXS）结果表明，与I型薄片相比，II型薄片显示出平均厚度和厚度分布宽度增加，非晶和结晶成分增加。尺寸排阻色谱法（SEC）数据主要显示了两种酶（i）和（ii），它们合成了支链淀粉。降低GBSSI或SBE活性会增加支链淀粉长链的数量（聚合度（DP）≥33）。
更新日期：2019-03-29
中文翻译：
下调生物合成酶的甘薯淀粉薄片中纳米链组装的变化。
颗粒结合的淀粉合酶I（GBSSI）和淀粉分支酶I和II（SBEI和SBEII）是至关重要的酶，可以生物合成具有不同表观直链淀粉含量和支链淀粉分支结构的淀粉。搭配红薯（番薯）[L.]林。简历。Xushu22），这项工作表明，下调GBSSI（对于蜡状淀粉）或SBE（对于高直链淀粉）活性，除了广泛报道的I型薄片之外，还可以在甘薯淀粉中形成新的半结晶薄片（命名为II型）。小角X射线散射（SAXS）结果表明，与I型薄片相比，II型薄片显示出平均厚度和厚度分布宽度增加，非晶和结晶成分增加。尺寸排阻色谱法（SEC）数据主要显示了两种酶（i）和（ii），它们合成了支链淀粉。降低GBSSI或SBE活性会增加支链淀粉长链的数量（聚合度（DP）≥33）。
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