Enzyme Reactor Based on Reversible pH-Controlled Catalytic Polymer Porous Membrane,ACS Applied Materials & Interfaces

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Enzyme Reactor Based on Reversible pH-Controlled Catalytic Polymer Porous Membrane
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-03-28 00:00:00 , DOI: 10.1021/acsami.9b01951
Juan Qiao _{1,

2} , Junfang Jiang _{1,

2} , Lili Liu _{1,

3} , Ji Shen _{1,

2} , Li Qi _{1,

2}

Affiliation

The challenge for polymeric enzyme reactors at present is to selectively control the enzymolysis rate in complex conditions. Additionally, the fabrication methodology is hindered by complex processes, especially for achieving diverse stimuli responsiveness and functions. Here, we reported a kind of pH-sensitive polymer, poly(styrene-co-maleic anhydride-acrylic acid) (PS-MAn-AA)-based hybrid enzyme reactor. It comprised magnetic nanoparticles and a pH-sensitive PS-MAn-AA porous polymer membrane made by breath figure method. The enzyme l-asparaginase (l-ASNase) could covalently bond on the surface of the pH-sensitive porous polymer membrane (pH-PPM), and the resultant enzyme reactor was characterized by Fourier transform infrared spectroscopy and vibrating sample magnetometer. The apparent Michaelis–Menten constants (K_m and V_max) of the l-ASNase enzyme reactor at different pH values were determined by a chiral ligand-exchange capillary electrophoresis method with l-asparagine as the substrate. The V_max value of the l-ASNase enzyme reactor (0.67 mM/min) was almost 3-fold of that of the free l-ASNase (0.23 mM/min) at pH 8.2. Its ability to precisely control the enzymolysis rate in complex conditions is triggered primarily by the pH of the buffer solution, allowing controlled enzymatic reactions and displaying excellent stability and reusability of the proposed pH-PPM. This strategy for porous polymer membrane enzyme reactor fabrication has established a platform for enzyme efficiency adjusting. These valve-like distinguished features highlight the outstanding potential of stimuli-responsive enzyme reactor applied for enzyme immobilization and enzyme-related disease treatment.

中文翻译：

基于可逆pH控制的催化聚合物多孔膜的酶反应器

当前对于聚合酶反应器的挑战是在复杂条件下选择性地控制酶解速率。另外，制造方法受到复杂过程的阻碍，特别是对于实现多种刺激响应和功能而言。在此，我们报道了一种pH敏感的聚合物，聚（苯乙烯-共-马来酸酐-丙烯酸）（PS-MAN-AA）为基础的混合酶反应器中。它由磁性纳米颗粒和通过呼吸图法制得的pH敏感型PS-MAn-AA多孔聚合物膜组成。酶l-天冬酰胺酶（l-ASNase）可以共价键合在pH敏感的多孔聚合物膜（pH-PPM）的表面上，并通过傅立叶变换红外光谱和振动样品磁强计对所得的酶反应器进行表征。用手性配体交换毛细管电泳法，以1-天冬酰胺为底物，测定在不同pH值下1- ASNase酶反应器的表观Michaelis-Menten常数（K _m和V _max）。1- ASNase酶反应器的V _max值（0.67 mM / min）几乎是游离I -ASNase反应器的V _max值的3倍。-ASNase（0.23 mM / min）在pH 8.2下。它在复杂条件下精确控制酶解速率的能力主要由缓冲溶液的pH值触发，从而可以实现受控的酶促反应，并显示出所提出的pH-PPM的出色稳定性和可重复使用性。这种用于多孔聚合物膜酶反应器制造的策略为酶效率调节建立了平台。这些类似阀门的突出特征突出了刺激响应酶反应器在酶固定和酶相关疾病治疗中的巨大潜力。

更新日期：2019-03-28

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