<br>将神经元输入映射到小鼠弓状核中的 Kiss1 神经元。,PLOS ONE

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将神经元输入映射到小鼠弓状核中的 Kiss1 神经元。
PLOS ONE ( IF 2.9 ) Pub Date : 2019-03-27 , DOI: 10.1371/journal.pone.0213927
Shel-Hwa Yeo ₁ , Victoria Kyle ₁ , Clemence Blouet ₂ , Susan Jones ₁ , William Henry Colledge ₁

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哺乳动物生殖轴的正常功能受到生理、代谢和环境因素的强烈影响。 Kisspeptin 神经肽由 Kiss1 基因编码，通过刺激下丘脑分泌促性腺激素释放激素，是哺乳动物生殖轴的有效调节剂。为了了解高阶神经元输入如何调节生殖轴，我们将传入回路映射到弓形 (ARC) Kiss1 神经元。我们使用在 Kiss1 神经元中表达 CRE 重组酶的转基因小鼠，使用转基因病毒进行条件病毒追踪。这些病毒在 Kiss1 神经元中由 CRE 介导的激活，使病毒能够跨突触移动，以标记具有初级或次级传入输入的神经元进入 Kiss1 神经元。大脑的几个区域显示出与弓形 Kiss1 神经元的突触连接，包括 ARC 本身的阿片黑皮素原神经元、前腹侧室周核中的 Kisspeptin 神经元、视上核和视交叉上核中的加压素神经元、室旁核中的促甲状腺激素释放神经元以及其他区域中的未识别神经元包括穹窿下器官、杏仁核、脚间核、腹侧前乳头核、终纹基底核以及皮质的视觉、体感和梨状区。这些数据让我们深入了解 Kiss1 神经元的活动如何受到代谢信号的调节，并为未来的功能研究提供详细的神经解剖图。

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Mapping neuronal inputs to Kiss1 neurons in the arcuate nucleus of the mouse.

The normal function of the mammalian reproductive axis is strongly influenced by physiological, metabolic and environmental factors. Kisspeptin neuropeptides, encoded by the Kiss1 gene, are potent regulators of the mammalian reproductive axis by stimulating gonadodropin releasing hormone secretion from the hypothalamus. To understand how the reproductive axis is modulated by higher order neuronal inputs we have mapped the afferent circuits into arcuate (ARC) Kiss1 neurons. We used a transgenic mouse that expresses the CRE recombinase in Kiss1 neurons for conditional viral tracing with genetically modified viruses. CRE-mediated activation of these viruses in Kiss1 neurons allows the virus to move transynaptically to label neurons with primary or secondary afferent inputs into the Kiss1 neurons. Several regions of the brain showed synaptic connectivity to arcuate Kiss1 neurons including proopiomelanocortin neurons in the ARC itself, kisspeptin neurons in the anteroventral periventricular nucleus, vasopressin neurons in the supraoptic and suprachiasmatic nuclei, thyrotropin releasing neurons in the paraventricular nucleus and unidentified neurons in other regions including the subfornical organ, amygdala, interpeduncular nucleus, ventral premammilary nucleus, basal nucleus of stria terminalis and the visual, somatosensory and piriform regions of the cortex. These data provide an insight into how the activity of Kiss1 neurons may be regulated by metabolic signals and provide a detailed neuroanatomical map for future functional studies.

更新日期：2019-03-28

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