Development of a Thiolysis HPLC Method for the Analysis of Procyanidins in Cranberry Products,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Development of a Thiolysis HPLC Method for the Analysis of Procyanidins in Cranberry Products
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-02-12 00:00:00 , DOI: 10.1021/acs.jafc.7b04625
Chi Gao ₁ , David G. Cunningham ₂ , Haiyan Liu ₂ , Christina Khoo ₂ , Liwei Gu ₁

Affiliation

The objective of this study was to develop a thiolysis HPLC method to quantify total procyanidins, the ratio of A-type linkages, and A-type procyanidin equivalents in cranberry products. Cysteamine was utilized as a low-odor substitute of toluene-α-thiol for thiolysis depolymerization. A reaction temperature of 70 °C and reaction time of 20 min, in 0.3 M of HCl, were determined to be optimum depolymerization conditions. Thiolytic products of cranberry procyanidins were separated by RP-HPLC and identified using high-resolution mass spectrometry. Standards curves of good linearity were obtained on thiolyzed procyanidin dimer A2 and B2 external standards. The detection and quantification limits, recovery, and precision of this method were validated. The new method was applied to quantitate total procyanidins, average degree of polymerization, ratio of A-type linkages, and A-type procyanidin equivalents in cranberry products. Results showed that the method was suitable for quantitative and qualitative analysis of procyanidins in cranberry products.

中文翻译：

蔓越莓产品中原花青素含量分析的硫解高效液相色谱方法的建立

这项研究的目的是开发一种硫解HPLC方法，以定量蔓越莓产品中的总原花青素，A型键的比率和A型原花青素当量。半胱胺被用作甲苯-α-硫醇的低气味替代物，用于硫解解聚。确定在70 M的HCl中70℃的反应温度和20分钟的反应时间是最佳的解聚条件。蔓越莓原花青素的硫解产物通过RP-HPLC分离，并使用高分辨率质谱进行鉴定。在硫解原花青素二聚体A2和B2外标上获得了线性良好的标准曲线。验证了该方法的检测和定量限，回收率和精密度。该新方法用于定量总原花青素，平均聚合度，蔓越莓产品中A型键的比例和A型原花青素当量的比率。结果表明该方法适用于蔓越莓产品中原花青素的定量和定性分析。

更新日期：2018-02-12

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南