An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments,Journal of the American Chemical Society

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An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2019-03-25 , DOI: 10.1021/jacs.8b10776
Gina L C Grammbitter ₁ , Maximilian Schmalhofer ₂ , Kudratullah Karimi ₃ , Yi-Ming Shi ₁ , Tim A Schöner ₁ , Nicholas J Tobias ₁ , Nina Morgner ₃ , Michael Groll ₂ , Helge B Bode ₁

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Aryl polyene (APE) pigments are a widely distributed class of bacterial polyketides. So far, little is known about the biosynthesis of these compounds, which are produced by a novel type II polyketide synthase (PKS). We have identified all enzymes involved in APE biosynthesis and determined their peculiar functions. The biosynthesis was reconstituted in vitro, and ACP-bound intermediates were assigned for each reaction step by HPLC-MS. Native mass spectrometry experiments identified four stable complexes: the acyl-carrier proteins ApeE and ApeF bound to the thioesterase ApeK, the dehydratases ApeI and ApeP, and the ketosynthase ApeO in complex with its chain-length factor ApeC. X-ray structures of the heterodimeric ApeO:ApeC and ApeI:ApeP complexes depict striking protein-protein interactions. Altogether, our study elucidated mechanistic aspects of APE biosynthesis that unifies elements of type II fatty acid and PKS systems, but in addition includes novel enzyme complexes.

中文翻译：

一种罕见的 II 型 PKS 催化芳基多烯颜料的生物合成

芳基多烯 (APE) 颜料是一类广泛分布的细菌聚酮化合物。到目前为止，对这些化合物的生物合成知之甚少，这些化合物是由新型 II 型聚酮化合物合酶 (PKS) 产生的。我们已经确定了参与 APE 生物合成的所有酶，并确定了它们的特殊功能。在体外重组生物合成，并通过 HPLC-MS 为每个反应步骤指定 ACP 结合的中间体。天然质谱实验确定了四种稳定的复合物：与硫酯酶 ApeK 结合的酰基载体蛋白 ApeE 和 ApeF、脱水酶 ApeI 和 ApeP，以及与其链长因子 ApeC 复合的酮合酶 ApeO。异二聚体 ApeO:ApeC 和 ApeI:ApeP 复合物的 X 射线结构描绘了惊人的蛋白质-蛋白质相互作用。共，

更新日期：2019-03-25

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