Abstract The objective of this work was to investigate the possibility of a macromolecular network formation in gluten-free model dough systems based on millet flours (white and wholemeal) by the addition of rye bran arabinoxylan (AX) and enzyme treatment. To perform enzyme treatment pyranose-2-oxidase was applied, which was assumed to be able to initiate cross-links between AX molecules through H2O2 production. The effect of AX addition and enzyme treatment on the rheological properties of the model systems was studied by basic (oscillation measurement) and applied (micro-doughLab, Rapid Visco Analyzer and Mixolab) rheological methods. The molecular basis of AX crosslinking was investigated in lyophilized dough and/or gel samples by SE-HPLC and Scanning Electron Microscopy (SEM). The results showed that AX addition deteriorated the mixing and pasting properties in general, but these properties were significantly improved when it was combined with enzyme treatment. In some cases, enzyme addition itself also changed the behaviour of the model systems significantly, suggesting that the enzyme may have an effect on other flour components (e.g. proteins, starch), as well. The study of AX molecular weight distribution performed by SE-HPLC confirmed the appearance of a new peak in the higher molecular weight range when enzyme treatment was applied to AX dosed dough systems, supporting our initial assumptions. According to SEM images of dough and gel microstructures, significant association was observed between starch granules when AX and enzyme treatment were applied together indicating a possible formation of a macromolecular network.

中文翻译：

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添加戊聚糖和酶处理对小米粉模型面团系统流变学特性的影响研究

摘要 这项工作的目的是通过添加黑麦麸阿拉伯木聚糖 (AX) 和酶处理，研究在基于小米面粉（白面粉和全麦面粉）的无麸质模型面团系统中形成大分子网络的可能性。使用吡喃糖-2-氧化酶进行酶处理，假设它能够通过产生 H2O2 来启动 AX 分子之间的交联。通过基本（振荡测量）和应用（微面团实验室、快速粘度分析仪和 Mixolab）流变方法研究了 AX 添加和酶处理对模型系统流变特性的影响。通过 SE-HPLC 和扫描电子显微镜 (SEM) 在冻干面团和/或凝胶样品中研究 AX 交联的分子基础。结果表明，AX的加入总体上使混合和糊化性能变差，但与酶处理相结合时，这些性能得到显着改善。在某些情况下，酶添加本身也显着改变了模型系统的行为，表明酶也可能对其他面粉成分（例如蛋白质、淀粉）产生影响。SE-HPLC 对 AX 分子量分布的研究证实，当酶处理应用于 AX 剂量面团系统时，在较高分子量范围内出现了一个新峰，支持我们的初步假设。根据面团和凝胶微观结构的 SEM 图像，