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High-throughput acoustofluidic fabrication of tumor spheroids.
Lab on a Chip ( IF 6.1 ) Pub Date : 2019-05-14 , DOI: 10.1039/c9lc00135b
Bin Chen 1 , Yue Wu , Zheng Ao , Hongwei Cai , Asael Nunez , Yunhua Liu , John Foley , Kenneth Nephew , Xiongbin Lu , Feng Guo
Affiliation  

Three-dimensional (3D) culture of multicellular spheroids, offering a desirable biomimetic microenvironment, is appropriate for recapitulating tissue cellular adhesive complexity and revealing a more realistic drug response. However, current 3D culture methods are suffering from low-throughput, poor controllability, intensive-labor, and variation in spheroid size, thus not ready for many high-throughput screening applications including drug discovery and toxicity testing. Herein, we developed a high-throughput multicellular spheroid fabrication method using acoustofluidics. By acoustically-assembling cancer cells with low-cost and disposable devices, our method can produce more than 12 000 multicellular aggregates within several minutes and allow us to transfer these aggregates into ultra-low attachment dishes for long-term culture. This method can generate more than 6000 tumor spheroids per operation, and reduce tumor spheroid formation time to one day. Our platform has advantages in forming spheroids with high throughput, short time, and long-term effectiveness, and is easy-to-operation. This acoustofluidic spheroid assembly method provides a simple and efficient way to produce large numbers of uniform-sized spheroids for biomedical applications in translational medicine, pharmaceutical industry and basic life science research.

中文翻译:

肿瘤球体的高通量声流制造。

提供理想的仿生微环境的多细胞球体的三维(3D)培养适用于概括组织细胞胶粘剂的复杂性并揭示更现实的药物反应。但是,当前的3D培养方法存在通量低,可控性差,劳动强度大以及球体尺寸变化的问题,因此还不能为包括药物发现和毒性测试在内的许多高通量筛选应用做好准备。本文中,我们开发了一种使用声流体技术的高通量多细胞球体制造方法。通过用低成本和一次性设备声学组装癌细胞,我们的方法可以在几分钟内产生超过12 000个多细胞聚集体,并使我们能够将这些聚集体转移到超低附着力培养皿中进行长期培养。该方法每次手术可产生6000多个肿瘤球体,并将肿瘤球体形成时间减少到一天。我们的平台具有形成高通量,短时间和长期有效性的球体的优点,并且易于操作。这种声流体球体组装方法提供了一种简单有效的方法,可生产大量均一尺寸的球体,用于转化医学,制药业和基础生命科学研究中的生物医学应用。
更新日期:2019-03-25
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