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Molecular Effects of Glycerol on Lipid Monolayers at the Gas-Liquid Interface: Impact on Microbubble Physical and Mechanical Properties.
Langmuir ( IF 3.7 ) Pub Date : 2019-03-22 00:00:00 , DOI: 10.1021/acs.langmuir.8b04130 Radwa H Abou-Saleh 1, 2 , James R McLaughlan 3, 4 , Richard J Bushby 5 , Benjamin R Johnson 1 , Steven Freear 3 , Stephen D Evans 1 , Neil H Thomson 1, 6
Langmuir ( IF 3.7 ) Pub Date : 2019-03-22 00:00:00 , DOI: 10.1021/acs.langmuir.8b04130 Radwa H Abou-Saleh 1, 2 , James R McLaughlan 3, 4 , Richard J Bushby 5 , Benjamin R Johnson 1 , Steven Freear 3 , Stephen D Evans 1 , Neil H Thomson 1, 6
Affiliation
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The production and stability of microbubbles (MBs) is enhanced by increasing the viscosity of both the formation and storage solution, respectively. Glycerol is a good candidate for biomedical applications of MBs, since it is biocompatible, although the exact molecular mechanisms of its action is not fully understood. Here, we investigate the influence glycerol has on lipid-shelled MB properties, using a range of techniques. Population lifetime and single bubble stability were studied using optical microscopy. Bubble stiffness measured by AFM compression is compared with lipid monolayer behavior in a Langmuir–Blodgett trough. We deduce that increasing glycerol concentrations enhances stability of MB populations through a 3-fold mechanism. First, binding of glycerol to lipid headgroups in the interfacial monolayer up to 10% glycerol increases MB stiffness but has limited impact on shell resistance to gas permeation and corresponding MB lifetime. Second, increased solution viscosity above 10% glycerol slows down the kinetics of gas transfer, markedly increasing MB stability. Third, above 10%, glycerol induces water structuring around the lipid monolayer, forming a glassy layer which also increases MB stiffness and resistance to gas loss. At 30% glycerol, the glassy layer is ablated, lowering the MB stiffness, but MB stability is further augmented. Although the molecular interactions of glycerol with the lipid monolayer modulate the MB lipid shell properties, MB lifetime continually increases from 0 to 30% glycerol, indicating that its viscosity is the dominant effect on MB solution stability. This three-fold action and biocompatibility makes glycerol ideal for therapeutic MB formation and storage and gives new insight into the action of glycerol on lipid monolayers at the gas–liquid interface.
中文翻译：
甘油对气液界面脂质单层的分子影响：对微泡物理和机械性能的影响。
微泡（MBs）的产生和稳定性分别通过增加地层溶液和储存溶液的粘度来提高。甘油由于具有生物相容性，因此是MBs生物医学应用的良好候选者，尽管对其作用的确切分子机理尚不完全清楚。在这里，我们使用一系列技术研究了甘油对带壳蛋壳MB性能的影响。使用光学显微镜研究了种群寿命和单泡稳定性。将通过AFM压缩测量的气泡刚度与Langmuir-Blodgett槽中的脂质单层行为进行了比较。我们推断增加甘油浓度可通过3倍机制增强MB种群的稳定性。第一的，甘油与界面单层中高达10％的甘油的脂质头基的结合增加了MB的刚度，但对壳层的抗气体渗透性和相应的MB寿命产生了有限的影响。第二，高于10％甘油的溶液粘度增加会减慢气体转移的动力学，显着提高MB的稳定性。第三，甘油含量超过10％时，会引起脂质单层周围的水结构化，形成玻璃状层，这也增加了MB的刚度和抗气体损失性。甘油含量为30％时，玻璃状层会被烧蚀，从而降低MB刚度，但MB稳定性会进一步提高。尽管甘油与脂质单分子层的分子相互作用调节了MB脂质的壳层性能，但MB的使用寿命从0％不断提高到30％，表明其粘度是MB溶液稳定性的主要影响因素。这种三重作用和生物相容性使甘油成为治疗性MB形成和储存的理想选择，并为甘油对气液界面脂质单层的作用提供了新的见解。
更新日期：2019-03-22
中文翻译：
甘油对气液界面脂质单层的分子影响：对微泡物理和机械性能的影响。
微泡（MBs）的产生和稳定性分别通过增加地层溶液和储存溶液的粘度来提高。甘油由于具有生物相容性，因此是MBs生物医学应用的良好候选者，尽管对其作用的确切分子机理尚不完全清楚。在这里，我们使用一系列技术研究了甘油对带壳蛋壳MB性能的影响。使用光学显微镜研究了种群寿命和单泡稳定性。将通过AFM压缩测量的气泡刚度与Langmuir-Blodgett槽中的脂质单层行为进行了比较。我们推断增加甘油浓度可通过3倍机制增强MB种群的稳定性。第一的，甘油与界面单层中高达10％的甘油的脂质头基的结合增加了MB的刚度，但对壳层的抗气体渗透性和相应的MB寿命产生了有限的影响。第二，高于10％甘油的溶液粘度增加会减慢气体转移的动力学，显着提高MB的稳定性。第三，甘油含量超过10％时，会引起脂质单层周围的水结构化，形成玻璃状层，这也增加了MB的刚度和抗气体损失性。甘油含量为30％时，玻璃状层会被烧蚀，从而降低MB刚度，但MB稳定性会进一步提高。尽管甘油与脂质单分子层的分子相互作用调节了MB脂质的壳层性能，但MB的使用寿命从0％不断提高到30％，表明其粘度是MB溶液稳定性的主要影响因素。这种三重作用和生物相容性使甘油成为治疗性MB形成和储存的理想选择，并为甘油对气液界面脂质单层的作用提供了新的见解。
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