OsIAAGLU 的过表达揭示了 IAA-葡萄糖结合在调节水稻植株结构中的作用,Plant Cell Reports

当前位置： X-MOL 学术 › Plant Cell Rep. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

OsIAAGLU 的过表达揭示了 IAA-葡萄糖结合在调节水稻植株结构中的作用
Plant Cell Reports ( IF 5.3 ) Pub Date : 2019-03-22 , DOI: 10.1007/s00299-019-02402-4
Xiao-Lu Yu ₁ , Hai-Yan Wang ₁ , David W M Leung ₂ , Zhi-Dan He ₁ , Jian-Jun Zhang ₁ , Xin-Xiang Peng ₁ , E-E Liu ₁

Affiliation

关键信息

OsIAAGLU可以催化 IAA 与葡萄糖反应生成 IAA-葡萄糖。OsIAAGLU在水稻中的过表达导致水稻地上部结构和根向地性的改变。

抽象的

众所周知，植物组织中吲哚-3-乙酸 (IAA) 的分布和水平在植物生长和发育中起着至关重要的作用。调节单子叶植物中游离 IAA 水平的一个重要机制是 IAA 酯缀合物的形成。在这项研究中，发现由水稻吲哚-3-乙酸葡糖基转移酶 ( OsIAAGLU ) 基因编码的胞质溶胶定位蛋白可催化游离 IAA 与葡萄糖反应生成 IAA-葡萄糖。OsIAAGLU的表达可由IAA 和NAA 诱导。与野生型（WT）植物相比，过表达OsIAAGLU的转基因水稻品系的分蘖数和叶角显着增加，同时株高和穗长减少。iaaglu的表型使用CRISPR/Cas9系统构建的突变体与WT植物没有明显差异。此外，OsIAAGLU的过表达导致转基因植物对 IAA/NAA 的敏感性降低，并改变了根的重力响应。过表达OsIAAGLU转基因株系的叶片、根尖和叶片关节中游离IAA含量低于WT植株。这些结果支持OsIAAGLU可以在 IAA 稳态和水稻结构中发挥调节作用。

"点击查看英文标题和摘要"

Overexpression of OsIAAGLU reveals a role for IAA–glucose conjugation in modulating rice plant architecture

Key message

OsIAAGLU could catalyze the reaction of IAA with glucose to generate IAA-glucose. Overexpression of OsIAAGLU in rice resulted in altered rice shoot architecture and root gravitropism.

Abstract

The distribution and levels of indole-3-acetic acid (IAA) within plant tissues are well known to play vital roles in plant growth and development. An important mechanism of regulating free IAA levels in monocots is formation of IAA ester conjugates. In this study, a cytosol-localized protein encoded by the rice gene of indole-3-acetic acid glucosyltransferase (OsIAAGLU) was found to catalyze the reaction of free IAA with glucose to generate IAA-glucose. Expression of OsIAAGLU could be induced by IAA and NAA. The number of tillers and leaf angle was significantly increased with a concomitant decrease in plant height and panicle length in the transgenic rice lines overexpressing OsIAAGLU compared to the wild-type (WT) plants. Phenotypes of iaaglu mutants constructed using the CRISPR/Cas9 system had no obvious differences with WT plants. Furthermore, overexpression of OsIAAGLU resulted in reduced sensitivity to IAA/NAA and altered gravitropic response of the roots in the transgenic plants. Free IAA contents in the leaves, root tips, and lamina joint of OsIAAGLU-overexpressing transgenic lines were lower than those of WT plants. These results support that OsIAAGLU could play a regulatory role in IAA homeostasis and rice architecture.

更新日期：2019-03-22

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南