Enhanced Electrochemical Activity of a Hollow Carbon Sphere/Polyaniline-Based Electrochemical Biosensor for HBV DNA Marker Detection,ACS Biomaterials Science & Engineering

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Enhanced Electrochemical Activity of a Hollow Carbon Sphere/Polyaniline-Based Electrochemical Biosensor for HBV DNA Marker Detection
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-03-21 00:00:00 , DOI: 10.1021/acsbiomaterials.8b01520
Razieh Salimian ₁ , Saeed Shahrokhian _{1,

2} , Saba Panahi ₁

Affiliation

Herein, we present a novel, simple, and ultrasensitive electrochemical DNA (E-DNA) sensor based on hollow carbon spheres (HCS) decorated with polyaniline (PANI). A thiolated 21-mer oligonucleotide, characteristic of HBV DNA, is immobilized via electrodeposited gold nanoparticles on HCS-PANI. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) are used to characterize the electrochemical properties of the prepared nanocomposite. Scanning electron microscopy is employed to investigate the morphological texture of the fabricated modifier. An enhanced intrinsic signal of PANI is probed to evaluate the biosensing ability of the prepared modifier. The proposed biosensor allows for the detection of the target sequences of HBV DNA at a concentration as low as 10 fM (i.e., 10⁹ DNA copies/mL). In addition, this biosensor demonstrated good capability to differentiate between the perfectly matched target oligonucleotide and three nucleotide-mismatched oligonucleotides. Furthermore, the HCS/PANI-based E-DNA sensor indicates highly sensitive detection of HBV DNA in real samples.

中文翻译：

空心碳球/聚苯胺基电化学生物传感器用于HBV DNA标记检测的增强的电化学活性

在这里，我们提出了一种新颖，简单，超灵敏的电化学DNA（E-DNA）传感器，该传感器基于装饰有聚苯胺（PANI）的中空碳球（HCS）。通过在HCS-PANI上电沉积的金纳米粒子固定了具有HBV DNA特征的21硫醇化寡核苷酸。循环伏安法（CV），差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）用于表征制备的纳米复合材料的电化学性质。扫描电子显微镜用于研究所制造的改性剂的形态织构。探寻增强的PANI固有信号，以评估所制备修饰剂的生物传感能力。提出的生物传感器允许以低至10 fM（即10 fM）的浓度检测HBV DNA的靶序列。^9个DNA拷贝/ mL）。另外，该生物传感器表现出良好的区分完全匹配的靶寡核苷酸和三个核苷酸错配的寡核苷酸的能力。此外，基于HCS / PANI的E-DNA传感器可对真实样品中的HBV DNA进行高度灵敏的检测。

更新日期：2019-03-21

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