Highly Selective Isomerization of Glucose into Fructose Catalyzed by a Mimic Glucose Isomerase,ChemCatChem

当前位置： X-MOL 学术 › ChemCatChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Highly Selective Isomerization of Glucose into Fructose Catalyzed by a Mimic Glucose Isomerase
ChemCatChem ( IF 3.8 ) Pub Date : 2019-04-02 , DOI: 10.1002/cctc.201900143
Ni Zhang ₁ , Xiang‐Guang Meng ₁ , Yan‐Yan Wu ₁ , Hong‐Jin Song ₁ , Hong Huang ₁ , Fei Wang ₁ , Jing Lv ₁

Affiliation

An amphoteric phenolic compound with carboxyl and amino functional groups was synthesized and used as mimic glucose isomerase to catalyze the isomerization of glucose into fructose. The mimic enzyme displayed excellent catalytic activity for isomerization of glucose to fructose under mild conditions. The yield of fructose and selectivity of fructose could reach 33.0 % and 93.5 % after reacting 4 h, respectively, at pH 8.5 and 80 °C. The catalysis reaction rate constant k_cat and Michaelis constants K_mG, K_mF were calculated. The activation energy of glucose to fructose was evaluated as 65.9 kJ mol⁻¹. The possible catalysis reaction mechanism was proposed. The acid‐base groups on the catalyst play an important role in proton extraction and transfer, as well as in the rotation of local carbon chain, which leads to the highly selective isomerization of glucose into fructose under mild conditions.

中文翻译：

模仿葡萄糖异构酶催化葡萄糖高选择性异构化为果糖

合成了具有羧基和氨基官能团的两性酚类化合物，并用作模拟葡萄糖异构酶，催化葡萄糖异构化为果糖。该模拟酶在温和条件下显示出优异的催化活性，可将葡萄糖异构化为果糖。在pH 8.5和80°C下反应4 h后，果糖的产率和果糖的选择性分别可以达到33.0％和93.5％。计算出催化反应速率常数k _cat和米氏常数K _mG，K _mF。葡萄糖对果糖的活化能评估为65.9 kJ mol ^-1。提出了可能的催化反应机理。催化剂上的酸基在质子的萃取和转移以及局部碳链的旋转中起着重要作用，这导致葡萄糖在温和条件下高度选择性地异构化为果糖。

更新日期：2019-04-02

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南