Journal of Proteomics ( IF 2.8 ) Pub Date : 2019-03-14 , DOI: 10.1016/j.jprot.2019.03.009 Zichao Liu 1 , Xiangtong Tong 1 , Yuan Su 1 , Debin Wang 1 , Xinru Du 1 , Fang Zhao 2 , Dingkang Wang 3 , Feng Zhao 2
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Medicinal leeches have been widely utilized in medical procedures for thousands of years. The application of leeches depends on the components of leech saliva secretions and active molecules, but many components of the secretions are not well characterized due to their low concentration and abundance. Determination of the profiles of leech salivary secretions is important to its medicinal application. In this study, we performed an in-depth proteomic analysis of leech salivary glands and deduced 434 full-length protein sequences from combined leech proteome and transcriptome databases. After integrating data from both datasets, forty-four proteins and two hundred twenty-one transcripts of bioactive molecules were involved in leech sucking pathways. Using gene expression analysis, we found that two-thirds of bioactive genes played key roles in leech bite processes and were associated cave-dwelling habitats. Our results indicate that the treatment efficiency can differ depending on the sucking leech species. Moreover, combining high-throughput proteomic and transcriptomic analyses is effective for the determination of wide profiles of proteins that are present at low concentrations in secretions. These findings highlight the extensive diversity of bioactive molecules and provide a new foundation for performing novel investigations and discovering future pharmacological agents or targets in leech medicinal therapy.
Significance
Medicinal leech therapy has been used for many centuries depending on the components of leech saliva secretions and active actions, but many components of the secretions were less known due to its low concentration and abundance. Determination of the profiles of leech salivary secretions is important to its medicinal application. Hereby, the molecular information provided by proteomic and transcriptomic analysis can be used to develop a more thorough understanding of leech sucking pathway and medicinal application. It provided a new foundation for performing novel investigations and discovering future pharmacological agents or targets in leech medicinal therapy.
中文翻译：
通过结合唾液腺蛋白质组和转录组数据确定水lee唾液分泌物中生物活性大分子的深入概况。
药用水ches已经在医疗程序中广泛使用了数千年。水的应用取决于水ech唾液分泌物和活性分子的成分，但是由于它们的低浓度和丰度，其分泌物的许多成分无法很好地表征。确定水sal唾液分泌物的概况对其医学应用很重要。在这项研究中，我们对水ech唾液腺进行了深入的蛋白质组学分析，并从水le蛋白质组和转录组数据库中推导了434个全长蛋白质序列。在整合了两个数据集的数据后，有44种蛋白质和212种生物活性分子的转录本参与了水suck的吮吸途径。使用基因表达分析，我们发现，三分之二的生物活性基因在水bit叮咬过程中起关键作用，并且与洞穴居所有关。我们的结果表明，治疗效率可能因吸吮的水ech种类而异。而且，将高通量蛋白质组学和转录组学分析相结合对于确定分泌物中低浓度存在的蛋白质的宽谱图是有效的。这些发现突出了生物活性分子的广泛多样性，并为进行新颖的研究和发现水ech药物治疗中的未来药理剂或靶标提供了新的基础。高通量蛋白质组学和转录组学分析相结合对于确定分泌物中低浓度存在的蛋白质的宽谱图有效。这些发现突出了生物活性分子的广泛多样性，并为进行新颖的研究和发现水ech药物治疗中的未来药理剂或靶标提供了新的基础。高通量蛋白质组学和转录组学分析相结合对于确定分泌物中低浓度存在的蛋白质的宽谱图有效。这些发现突出了生物活性分子的广泛多样性，并为进行新颖的研究和发现水ech药物治疗中的未来药理剂或靶标提供了新的基础。
意义
取决于水ech唾液分泌物的成分和主动作用，药用水therapy疗法已经使用了多个世纪，但由于其浓度低和丰度高，分泌物的许多成分鲜为人知。确定水sal唾液分泌物的概况对其医学应用很重要。因此，蛋白质组学和转录组学分析提供的分子信息可用于发展对水ech吸吮途径和医学应用的更全面的了解。它为进行新颖的研究和发现水ech药物治疗中的未来药理剂或靶标提供了新的基础。
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