64Cu-Based Pretargeted Immuno-Positron Emission Tomography and Near-Infrared Fluorescence Imaging of the Vascular Endothelial Growth Factor,ACS Omega

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64Cu-Based Pretargeted Immuno-Positron Emission Tomography and Near-Infrared Fluorescence Imaging of the Vascular Endothelial Growth Factor
ACS Omega ( IF 3.7 ) Pub Date : 2019-03-14 00:00:00 , DOI: 10.1021/acsomega.9b00158
Xudong Shi ₁ , Kai Gao ₁ , Ran Gao ₁

Affiliation

Pretargeted immuno-positron emission tomography (PET)/near-infrared fluorescence (NIRF) using bioorthogonal chemistry between radioisotope- or fluorophore-labeled Reppe anhydrides and vascular endothelial growth factor (VEGF)-specific monoclonal antibody bevacizumab–tetrazine conjugates was developed. The pretargeting approach provided a high target-to-background contrast. An in vivo investigation clearly indicated the suitability of the ⁶⁴Cu-radiolabeled Reppe anhydride for pretargeted PET imaging of VEGF-expressing colorectal tumors. The radiation exposure to healthy tissues decreased as a result of the rapid elimination of the PET probe. Pretargeted NIRF imaging was also used to visualize the VEGF expression in HCT116 tumors using bevacizumab–tetrazine/cyanine 5-labeled Reppe anhydride chemistry.

中文翻译：

血管内皮生长因子的基于⁶⁴ Cu的预靶向免疫正电子发射断层扫描和近红外荧光成像

使用放射性同位素或荧光团标记的Reppe酸酐与血管内皮生长因子（VEGF）特异性单克隆抗体贝伐单抗-四嗪结合物之间的生物正交化学方法，开发了预靶向免疫正电子发射断层扫描（PET）/近红外荧光（NIRF）。预定位方法提供了较高的目标到背景对比度。体内研究清楚地表明⁶⁴ Cu放射性标记的Reppe酸酐适合于表达VEGF的结直肠肿瘤的预靶向PET成像。由于快速消除了PET探针，减少了对健康组织的辐射暴露。使用贝伐单抗-四嗪/花青5标记的Reppe酸酐化学试剂，预先靶向的NIRF成像也可用于可视化HCT116肿瘤中的VEGF表达。

更新日期：2019-03-14

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