Designing Melittin-Graphene Hybrid Complexes for Enhanced Antibacterial Activity.,Advanced Healthcare Materials

当前位置： X-MOL 学术 › Adv. Healthcare Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Designing Melittin-Graphene Hybrid Complexes for Enhanced Antibacterial Activity.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2019-03-13 , DOI: 10.1002/adhm.201801521
Xuemei Lu ₁ , Jiaojiao Liu ₁ , Lu Gou ₂ , Jingliang Li ₃ , Bing Yuan ₁ , Kai Yang ₁ , Yuqiang Ma _{1,

4}

Affiliation

Antimicrobial peptides (AMPs) promise a fundamental solution to the devastating threat of drug-resistant bacteria. However, drawbacks of AMPs (e.g., poor cell membrane penetration efficiency) seriously block their clinical use. In this work, rational design of a hybrid complex of melittin (as a representative AMP) and graphene or graphene oxide (Gra or GO) nanosheets for enhanced antibacterial ability is achieved, via combining in-silico prediction and in-tube test. In comparison to pristine melittin, the specifically designed AMP-Gra (/GO) complex exhibits remarkable efficiency in transmembrane perforation with an over tenfold decrease in the threshold working concentration of peptide; moreover, it has an up to 20-fold enhancement in antibacterial activity against both Gram-negative and Gram-positive bacteria. Such improvement is ascribed to the synergetic insertion of nanosheets and melittin due to similarity in antibacterial mechanism between them and is further regulated by the structural factors of the complex, including the intersheet spacing and surface functionalization of the Gra/GO sheets, etc. These results provide practical guidelines to engineer AMPs with nanotechnology for improved antimicrobial performances, especially based on targeted functionalization of the Gra/GO nanosheets.

中文翻译：

设计用于增强抗菌活性的蜂毒素-石墨烯杂化复合物。

抗菌肽（AMPs）有望为抗药性细菌的毁灭性威胁提供基本解决方案。但是，AMPs的缺点（例如不良的细胞膜穿透效率）严重阻碍了其临床应用。在这项工作中，通过结合硅胶预测和管内测试，可以合理设计蜂毒肽（代表AMP）和石墨烯或氧化石墨烯（Gra或GO）纳米片的复合复合物，以增强抗菌能力。与原始蜂毒肽相比，专门设计的AMP-Gra（/ GO）复合物在跨膜穿孔中显示出显着的效率，肽的阈值工作浓度降低了十倍以上。此外，它对革兰氏阴性和革兰氏阳性细菌的抗菌活性提高了20倍。

更新日期：2019-03-13

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南