A simple, convenient, and inexpensive method to fabricate optical fiber based biosensors which utilize periodic hole arrays in gold films for signal transduction is reported. The process of hole array formation mainly relies on self-assembly of hydrogel microgels in combination with chemical gold film deposition and subsequent transfer of the perforated film onto an optical fiber tip. In the fabrication process solely chemical wet lab techniques are used, avoiding cost-intensive instrumentation or clean room facilities. The presented method for preparing fiber optic plasmonic sensors provides high throughput and is perfectly suited for commercialization using batch processing. The transfer of the perforated gold film onto an optical fiber tip does not affect the sensitivity of the biosensor ((420 ± 83) nm/refractive index unit (RIU)), which is comparable to sensitivities of sensor platforms based on periodic hole arrays in gold films prepared by significantly more complex methods. Furthermore, real-time and in-line immunoassay studies with a specially designed 3D printed flow cell are presented exploiting the presented optical fiber based biosensors.

报道了一种简单，方便且廉价的方法来制造基于光纤的生物传感器，该传感器利用金膜中的周期性孔阵列进行信号转导。孔阵列形成的过程主要依赖于水凝胶微凝胶的自组装，结合化学金膜的沉积以及随后将穿孔膜转移到光纤尖端上的过程。在制造过程中，仅使用化学湿法实验室技术，避免了成本高昂的仪器或无尘室设施。所提出的用于制备光纤等离子体传感器的方法提供了高通量，并且非常适合使用批处理进行商业化。穿孔金膜转移到光纤尖端上不会影响生物传感器的灵敏度（（420±83）nm /折射率单位（RIU）），这可与基于通过明显更复杂的方法制备的金膜中的周期性孔阵列的传感器平台的灵敏度相媲美。此外，利用所提出的基于光纤的生物传感器，提出了利用专门设计的3D打印流通池进行的实时和在线免疫分析研究。