A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels†,RSC Advances

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A lysosome-targetable fluorescent probe for the simultaneous sensing of Cys/Hcy and GSH from different emission channels†
RSC Advances ( IF 3.9 ) Pub Date : 2019-03-12 00:00:00 , DOI: 10.1039/c9ra00210c
Hui Zhang _{1,

2} , Lizhen Xu ₁ , Wenxiu Li ₃ , Wenqiang Chen ₁ , Qi Xiao ₁ , Jun Huang ₁ , Chusheng Huang ₁ , Jiarong Sheng ₁ , Xiangzhi Song ₂

Affiliation

A lysosome-specific fluorescent probe, Lyso-AC, for biothiols was developed by incorporation of a 4-nitrophenol moiety into a coumarin dye. The Cys/Hcy-triggered substitution-rearrangement cascade, and GSH-induced substitution reaction lead to the corresponding blue emissive amino-coumarin and yellow emissive thiol-coumarin, thereby enabling Cys/Hcy and GSH detection from distinct emissions. Moreover, this probe displayed an excellent lysosome targeting property with a 0.92 Pearson's colocalization coefficient by using Neutral Red as a reference. Significantly, biological experiments indicated Lyso-AC has the potential to monitor lysosome Cys/Hcy and GSH simultaneously in living HeLa cells from distinct emissions.

中文翻译：

溶酶体靶向荧光探针，用于同时检测来自不同发射通道的 Cys/Hcy 和 GSH†

通过将 4-硝基苯酚部分掺入香豆素染料中，开发了一种用于生物硫醇的溶酶体特异性荧光探针Lyso-AC 。 Cys/Hcy 触发的取代重排级联和 GSH 诱导的取代反应产生相应的蓝色发射氨基香豆素和黄色发射硫醇香豆素，从而能够从不同的发射中检测 Cys/Hcy 和 GSH。此外，以中性红为参考，该探针表现出优异的溶酶体靶向特性，皮尔逊共定位系数为0.92。值得注意的是，生物实验表明Lyso-AC有潜力同时监测活 HeLa 细胞中不同发射的溶酶体 Cys/Hcy 和 GSH。

更新日期：2019-03-12

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