Rauvolfia nukuhivensis is a well-known plant used for its wide range of beneficial effects in Marquesas islands. It is made up of diverse indole alkaloids and is used as traditional medicine for skin application. The actual mechanism behind the virtue of this plant is still unknown. Hence, in this study we aimed at deciphering the impact of R. nukuhivensis on skin immune system in context of (1) homeostasis, (2) pathogen infection and (3) inflammation. Here we show that R. nukuhivensis enhances cellular metabolic activity and wound healing without inducing cellular stress or disturbing cellular homeostasis. It reinforces the epithelial barrier by up-regulating hBD-1. Nevertheless, in pathogenic stress, R. nukuhivensis acts by preparing the immune system to be reactive and effective directly. Indeed, it enhances the innate immune response by increasing pathogens sensors such as TLR5. Finally, R. nukuhivensis blocks IL-22 induced hyperproliferation via PTEN and Filaggrin up-regulation as well as BCL-2 downregulation. In conclusion, this study provides evidence on the several cutaneous application potentials of R. nukuhivensis such as boosting the immune response or in restoring the integrity of the epithelial barrier.

努库希文萝芙木 (Rauvolfia nukuhivensis)是一种著名的植物，在马克萨斯群岛具有广泛的有益作用。它由多种吲哚生物碱组成，用作皮肤涂抹的传统药物。这种植物的功效背后的实际机制仍然未知。因此，在这项研究中，我们的目的是破译R的影响。 nukuhivensis在以下情况下对皮肤免疫系统的影响：(1) 体内平衡，(2) 病原体感染和 (3) 炎症。在这里我们证明R . nukuhivensis增强细胞代谢活动和伤口愈合，而不诱导细胞应激或扰乱细胞稳态。它通过上调 hBD-1 来增强上皮屏障。然而，在致病胁迫下， R .努库希文西斯通过使免疫系统做好直接反应和有效的准备来发挥作用。事实上，它通过增加 TLR5 等病原体传感器来增强先天免疫反应。最后， R . nukuhivensis通过上调 PTEN 和 Filaggrin 以及下调 BCL-2 来阻断 IL-22 诱导的过度增殖。总之，本研究提供了R的多种皮肤应用潜力的证据。 nukuhivensis，例如增强免疫反应或恢复上皮屏障的完整性。