Carbon dots on V2O5 nanowires are a viable peroxidase mimic for colorimetric determination of hydrogen peroxide and glucose,Microchimica Acta

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Carbon dots on V2O5 nanowires are a viable peroxidase mimic for colorimetric determination of hydrogen peroxide and glucose
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-03-11 , DOI: 10.1007/s00604-019-3344-6
Fatemeh Honarasa ₁ , Fatemeh Hosseini Kamshoori ₁ , Shiva Fathi ₁ , Zeinab Motamedifar ₁

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AbstractA nanocomposite was hydrothermally prepared from C-dots and V2O5 nanowires, and characterized by TEM, FTIR and XRD. Due to the synergistic effects between C-dots and V2O5 nanowires, the nanocomposite is found to possess peroxidase-mimicking activity. This finding was exploited to design colorimetric methods for determination of H2O2 and glucose (via glucose oxidase) by using of 3,3′,5,5′-tetramethylbenzidine (TMB) as the chromogenic substrate. The C-dot/V2O5 nanocomposite catalyzes hydrogen peroxide to oxidize TMB and the resultant product, i.e., TMB* produces a blue color in the solution. Also for glucose determination, at first glucose reacts with dissolved oxygen in the presence of glucose oxidase and generates H2O2. Then, produced H2O2 was monitored by the C-dot/V2O5 nanozyme in the presence of TMB. Intensity of the blue color in the solution at wavelength of 650 nm is an indication of H2O2 or glucose concentration. The response to H2O2 is linear in the 0.5–520 μM concentration ranges, and that for glucose from 0.7 μM to 300 μM. Graphical abstractSchematic presentation of peroxidase mimicking activity of C-dot/V2O5 nanocomposite and its application as sensitive colorimetric H2O2/glucose assay by using of 3,3′,5,5′-tetramethylbenzidine (TMB) as chromogenic substrate to induce a typical blue color reaction.

中文翻译：

V2O5 纳米线上的碳点是一种可行的过氧化物酶模拟物，用于比色测定过氧化氢和葡萄糖

摘要以碳点和 V2O5 纳米线为原料，通过水热法制备了一种纳米复合材料，并通过 TEM、FTIR 和 XRD 对其进行了表征。由于碳点和 V2O5 纳米线之间的协同作用，纳米复合材料被发现具有过氧化物酶模拟活性。利用这一发现设计了使用 3,3',5,5'-四甲基联苯胺 (TMB) 作为显色底物测定 H2O2 和葡萄糖（通过葡萄糖氧化酶）的比色方法。C-dot/V2O5 纳米复合材料催化过氧化氢氧化 TMB，所得产物，即 TMB* 在溶液中产生蓝色。同样对于葡萄糖测定，首先葡萄糖在葡萄糖氧化酶的存在下与溶解氧反应并生成 H2O2。然后，在 TMB 存在下，通过 C-dot/V2O5 纳米酶监测产生的 H2O2。溶液中波长为 650 nm 的蓝色强度表示 H2O2 或葡萄糖浓度。对 H2O2 的响应在 0.5-520 μM 浓度范围内呈线性，而对葡萄糖的响应在 0.7 μM 至 300 μM 之间。图形摘要 C-dot/V2O5 纳米复合材料的过氧化物酶模拟活性的示意图及其在敏感比色 H2O2/葡萄糖测定中的应用，使用 3,3',5,5'-四甲基联苯胺 (TMB) 作为显色底物诱导典型的蓝色反应。

更新日期：2019-03-11

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