Chiral Shell Core–Satellite Nanostructures for Ultrasensitive Detection of Mycotoxin,Small

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Chiral Shell Core–Satellite Nanostructures for Ultrasensitive Detection of Mycotoxin
Small ( IF 13.0 ) Pub Date : 2018-02-09 , DOI: 10.1002/smll.201703931
Jiarong Cai _{1,

2,

3} , Changlong Hao _{1,

2,

3} , Maozhong Sun _{1,

2,

3} , Wei Ma _{1,

2,

3} , Chuanlai Xu _{1,

2,

3} , Hua Kuang _{1,

2,

3}

Affiliation

Herein, the design of a DNA‐based chiral biosensor is described utilizing the self‐assembly of shell core–gold (Au) satellite nanostructures for the detection of mycotoxin, ochratoxin A (OTA). The assembly of core–satellite nanostructures based on OTA‐aptamer binding exhibits a strong chiral signal with an intense circular dichroism (CD) peak. The integrity of the assembly of core–satellite nanostructures is limited to some extent in the presence of different levels of OTA. Correspondingly, the chiral intensity of assembly is weakened with increasing OTA concentrations, allowing quantitative determination of the target. The developed chiral sensor shows an excellent linear relationship between the CD signal and concentrations of OTA in the range of 0.1–5 pg mL⁻¹ with a limit of detection as low as 0.037 pg mL⁻¹. The effectiveness of the biosensor in a sample of red wine is verified and a good recovery rate is obtained. These results suggest that the strategy has great potential for practical application.

中文翻译：

手性壳核–卫星纳米结构用于霉菌毒素的超灵敏检测

在此，描述了一种基于DNA的手性生物传感器的设计，该技术利用壳核金（Au）卫星纳米结构的自组装来检测霉菌毒素，ra曲霉毒素A（OTA）。基于OTA-适体结合的核心-卫星纳米结构的组装表现出强烈的手性信号，并带有强烈的圆二色性（CD）峰。在存在不同水平的OTA的情况下，核心-卫星纳米结构组装的完整性受到一定程度的限制。相应地，组装的手性强度随着OTA浓度的增加而减弱，从而可以定量测定目标。发达的手性传感器显示CD信号与OTA浓度在0.1–5 pg mL ^-1范围内具有极好的线性关系，检出限低至0.037 pg mL^-1。验证了生物传感器在红酒样品中的有效性，并获得了良好的回收率。这些结果表明该策略具有很大的实际应用潜力。

更新日期：2018-02-09

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