Novel Mussel-Inspired Universal Surface Functionalization Strategy: Protein-Based Coating with Residue-Specific Post-Translational Modification in Vivo,ACS Applied Materials & Interfaces

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Novel Mussel-Inspired Universal Surface Functionalization Strategy: Protein-Based Coating with Residue-Specific Post-Translational Modification in Vivo
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-03-07 00:00:00 , DOI: 10.1021/acsami.8b22551
Haishan Qi ₁ , Weiwei Zheng ₁ , Chen Zhang ₁ , Xiao Zhou ₁ , Lei Zhang _{1,

2}

Affiliation

Surface functionalization can effectively endow materials with desirable properties, promoting the performance between the material and environment, with extensive applications. However, a universal and straightforward surface functionalization method with biocompatibility is scarce. In this study, with synthetic biology strategy, recombinant mussel plaque protein with a zwitterionic peptide inspired by molecular chaperone was engineered through post-translational modification, in which 3,4-dihydroxyphenylalanine was residue-specifically obtained efficiently from tyrosine with tyrosinase coexpressed in vivo. The rational designed chimeric protein coating in this work could successfully anchor to various substrates and exhibit excellent antifouling performance in resisting protein adsorption, cell attachment, and bacterial adhesion with eminent biocompatibility.

中文翻译：

新颖的贻贝启发的通用表面功能化策略：体内残基特定的翻译后修饰的蛋白质基涂层。

表面功能化可以有效地赋予材料所需的特性，并在广泛的应用中促进材料与环境之间的性能。然而，缺乏具有生物相容性的通用且直接的表面官能化方法。在这项研究中，采用合成生物学策略，通过翻译后修饰设计了具有分子伴侣激发的两性离子肽的重组贻贝斑蛋白，其中从酪氨酸和酪氨酸酶体内共表达的3,4-二羟基苯丙氨酸残基特异性地有效获得。在这项工作中设计合理的嵌合蛋白涂层可以成功地锚定到各种基质上，并在抵抗蛋白吸附，细胞附着，

更新日期：2019-03-07

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