使用一系列金纳米颗粒修饰的碳电极，用于与中东呼吸综合征相关的冠状病毒的电化学免疫传感器,Microchimica Acta

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使用一系列金纳米颗粒修饰的碳电极，用于与中东呼吸综合征相关的冠状病毒的电化学免疫传感器
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-03-07 , DOI: 10.1007/s00604-019-3345-5
Laila Ali Layqah , Shimaa Eissa

摘要中东呼吸综合征冠状病毒（MERS-CoV）具有高致病性。这里描述了一种用于确定 MERS-CoV 的免疫传感器。它基于在用金纳米粒子修饰的碳电极 (DEP) 阵列上进行的竞争性分析。重组刺突蛋白 S1 被用作 MERS CoV 的生物标志物。电极阵列能够对不同的 CoV 进行多重检测。生物传感器基于样品中的游离病毒和固定化的 MERS-CoV 蛋白之间的间接竞争，以将固定浓度的抗体添加到样品中。在添加不同浓度的针对 MERS-CoV 的抗原后，通过监测峰值电流（通常在 -0.05 V 相对于 Ag/AgCl 的工作电位下获得）的变化来检测伏安响应。使用方波伏安法 (SWV) 记录使用亚铁氰化物/铁氰化物作为探针的电化学测量。对于 MERS-CoV 和 HCoV，分别观察到传感器响应与 0.001 至 100 ng.mL-1 和 0.01 至 10,000 ng.mL-1 浓度之间的良好线性响应。该测定在 20 分钟内进行，HCoV 和 MERS-CoV 的检测限分别低至 0.4 和 1.0 pg.mL-1。该方法对流感 A 和 B 等非特异性蛋白质具有高度选择性。该方法是单步、灵敏且准确的。它已成功应用于加标鼻样本。图形摘要描述了中东呼吸综合征冠状病毒 (MERS-CoV) 的电化学免疫分析。该方法基于对具有金纳米粒子的纳米结构的碳阵列电极 (DEP) 进行的竞争性分析。阵列电极能够多重检测不同类型的冠状病毒。

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An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes

AbstractThe Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and immobilized MERS-CoV protein for a fixed concentration of antibody added to the sample. Voltammetric response is detected by monitoring the change in the peak current (typically acquired at a working potential of −0.05 V vs. Ag/AgCl) after addition of different concentrations of antigen against MERS-CoV. Electrochemical measurements using ferrocyanide/ferricyanide as a probe were recorded using square wave voltammetry (SWV). Good linear response between the sensor response and the concentrations from 0.001 to 100 ng.mL−1 and 0.01 to 10,000 ng.mL−1 were observed for MERS-CoV and HCoV, respectively. The assay was performed in 20 min with detection limit as low as 0.4 and 1.0 pg.mL−1 for HCoV and MERS-CoV, respectively. The method is highly selective over non-specific proteins such as Influenza A and B. The method is single-step, sensitive and accurate. It was successfully applied to spiked nasal samples. Graphical abstractAn electrochemical immunoassay is described for the Middle East Respiratory Syndrome Corona Virus (MERS-CoV). The method is based on a competitive assay carried out on a carbon array electrodes (DEP) nanostructured with gold nanoparticles. The array electrodes enable the multiplexed detection of different types of Corona Virus.

更新日期：2019-03-07

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