To mimic the mineral component and the microstructure of natural bone, nanohydroxyapatite (nHAP)/polymer (natural and synthetic materials) composite scaffolds with micropore structures and good mechanical performance were prepared via the assistance of sonication and amidation. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) showed the biomimetic homogeneous formation of nHAP in the polymer matrix. Fourier transform infrared spectroscopy (FTIR) indicated that the polymer matrix regulated the crystallization of nHAP. The integration of synthetic/natural polymers benefited nHAP crystallization in the polymer matrix and thus improved the mechanical performance of the chitosan (CS)/collagen (Col)/ poly (lactic acid) (PLA)/nHAP scaffolds. The in vitro bioactivity ability was investigated by incubating in a simulated body fluid (SBF). The methyl thiazolyl tetrazolium (MTT) test, the acridine orange/ethidium bromide (AO/EB) and Giemsa staining showed that the scaffolds had good biocompatibility and could maintain the cell growth. Therefore, the CS/Col/PLA/nHAP scaffold is promising for bone tissue engineering applications.

模仿矿物组分与天然骨的微观结构，制备了纳米羟基磷灰石（纳米羟基磷灰石）/聚合物（天然和合成材料）用微孔结构和良好的机械性能复合支架通过超声处理和酰胺化的协助。扫描电子显微镜（SEM）和X射线衍射（XRD）显示了在聚合物基质中仿生均匀形成nHAP。傅立叶变换红外光谱（FTIR）表明，聚合物基质调节nHAP的结晶。合成/天然聚合物的整合有利于nHAP在聚合物基质中的结晶，因此改善了壳聚糖（CS）/胶原蛋白（Col）/聚乳酸（PLA）/ nHAP支架的机械性能。该在体外通过在模拟体液（SBF）中孵育来研究生物活性。甲基噻唑基四唑鎓（MTT）测试，the啶橙/溴化乙锭（AO / EB）和Giemsa染色显示支架具有良好的生物相容性并可以维持细胞生长。因此，CS / Col / PLA / nHAP支架有望用于骨组织工程应用。