冷冻干燥工艺变量对明胶基支架的微观结构和性能的影响,Journal of Applied Polymer Science

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冷冻干燥工艺变量对明胶基支架的微观结构和性能的影响
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2019-02-23 , DOI: 10.1002/app.47671
V. Perez‐Puyana ₁ , M. Felix ₂ , A. Romero ₃ , A. Guerrero ₂

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在过去的几年中，组织工程领域经历了指数级增长，尽管加工参数确实会影响支架的特性，但只有少数研究调查了这一说法。因此，本研究分析了冷冻干燥过程中涉及的不同参数（容器，冷冻温度，pH和使用的溶剂）对明胶基支架的机械和结构性能的影响。因此，进行流变学测量和孔隙率分析以评估获得的支架。结果表明所评估的参数改变了脚手架的机械性能，突显了塑料模具选择包含使用低浓度（0.05 M）的弱酸（乙酸）作为溶剂生产的酸性蛋白质溶液的选择。相反，仅pH和冷冻温度导致这些支架的孔隙率显着不同，对于所有研究的系统，其值均高于95％。这些结果可用于证明加工技术中暗含的不同参数的控制允许设计具有适用于不同应用的特定性质的支架。分级为4 +©2019 Wiley Periodicals，Inc.J.Appl。Polym。科学分级为4 +©2019 Wiley Periodicals，Inc.J.Appl。Polym。科学分级为4 +©2019 Wiley Periodicals，Inc.J.Appl。Polym。科学2019，136，47671。

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Influence of the processing variables on the microstructure and properties of gelatin‐based scaffolds by freeze‐drying

In the last few years, the field of tissue engineering has suffered an exponential growth, and although it is true that the processing parameters affect the properties of the scaffolds, only a few studies have investigated that statement. For that reason, the influence of different parameters involved in the freeze‐drying process (container, freezing temperature, pH, and solvent used) on the mechanical and structural properties of gelatin‐based scaffolds was analyzed in this study. Thus, rheological measurements and porosity analyses were performed to evaluate the scaffolds obtained. Results indicate that the parameters evaluated modify the mechanical properties of the scaffold, highlighting the option of a plastic mold to contain an acidic protein solution produced using a weak acid (acetic acid) at low concentration (0.05 M) as solvent. On the contrary, only the pH and the freezing temperature led to significant differences in the porosity of these scaffolds, obtaining values higher than 95% for all the systems studied. These results are useful to demonstrate that the control of the different parameters implied in the processing technique allows designing a scaffold with specific properties suitable for different applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47671.

更新日期：2019-02-23

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