Integrated Lipidomics and Transcriptomics Characterization upon Aging-Related Changes of Lipid Species and Pathways in Human Bone Marrow Mesenchymal Stem Cells.,Journal of Proteome Research

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Integrated Lipidomics and Transcriptomics Characterization upon Aging-Related Changes of Lipid Species and Pathways in Human Bone Marrow Mesenchymal Stem Cells.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-04-05 , DOI: 10.1021/acs.jproteome.8b00936
Xin Lu ₁ , Yue Chen ₂ , Huiyu Wang ₃ , Yunfan Bai ₂ , Jianxiang Zhao ₂ , Xiaohan Zhang ₂ , Li Liang ₁ , Yang Chen ₁ , Chenfei Ye ₁ , Yiqun Li ₂ , Yi Zhang ₄ , Yu Li ₂ , Ting Ma _{1,

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Affiliation

Aberrant differentiations of bone mesenchymal stem cells (BMSCs) have proved to be associated with the occurrence of senile osteoporosis. However, mechanisms of this phenomenon relative to abnormal lipid metabolism remain unclear. This study was conducted to characterize the lipidomics alterations during BMSC passaging, aiming at uncovering the aging-related lipid metabolism that may play an important role in aberrant differentiations of BMSCs. Principal component analysis presented the sequential lipidomics alterations during BMSC passaging. The majority of glycerophospholipids, including phosphatidylcholines, phosphatidylethanolamines, phosphatidylglycerols, as well as sphingolipids, revealed significant elevations, whereas the others, including phosphatidic acids, phosphatidylinositols, and phosphatidylserines, presented decreases in aged cells. Double-bond equivalent versus carbon number plots demonstrated that the changing trends and significances of lipids during passaging were associated with the chain length and the degree of unsaturation. In the correlation networks, the scattering patterns of lipid categories suggested the category-related metabolic independence and potential conversion among phosphatidic acids, phosphatidylinositols, and phosphatidylserines. The lipid-enzyme integrated pathway analysis indicated the activated metabolic conversion from phosphatidic acids to CDP-diacylglycerol to phosphatidylinositols and from sphingosine to ceramides to sphingomyelins with BMSC passaging. The conversions among lipid species described the lipidomics responses that potentially induced the aberrant differentiations during BMSC aging.

中文翻译：

在人类骨髓间充质干细胞中脂质种类和途径的衰老相关变化后的综合脂质组学和转录组学表征。

骨间充质干细胞（BMSCs）的异常分化已被证明与老年性骨质疏松症的发生有关。但是，这种现象与脂质代谢异常有关的机制仍不清楚。进行这项研究以表征BMSC传代过程中的脂质组学变化，旨在揭示与衰老相关的脂质代谢，这些代谢可能在BMSCs的异常分化中起重要作用。主成分分析显示了BMSC传代过程中的顺序脂质组学改变。大多数衰老细胞中的甘油磷脂，包括磷脂酰胆碱，磷脂酰乙醇胺，磷脂酰甘油以及鞘脂显示出明显的升高，而其他磷脂酰磷脂，磷脂酰肌醇和磷脂酰丝氨酸则降低。双键当量对碳数图表明，传代过程中脂质的变化趋势和重要性与链长和不饱和度有关。在相关网络中，脂质类别的散射模式表明类别相关的代谢独立性以及磷脂酸，磷脂酰肌醇和磷脂酰丝氨酸之间的潜在转化。脂质-酶整合途径分析表明，通过BMSC传代，从磷脂酸到CDP-二酰基甘油到磷脂酰肌醇以及从鞘氨醇到神经酰胺再到鞘磷脂的代谢活化被激活。脂质种类之间的转换描述了可能在BMSC老化过程中引起异常分化的脂质组学响应。

更新日期：2019-04-07

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