The generation of new blood vessels via angiogenesis is critical for meeting tissue oxygen demands. A role for adult stem cells in this process remains unclear. Here, we identified CD157 (bst1, bone marrow stromal antigen 1) as a marker of tissue-resident vascular endothelial stem cells (VESCs) in large arteries and veins of numerous mouse organs. Single CD157⁺ VESCs form colonies in vitro and generate donor-derived portal vein, sinusoids, and central vein endothelial cells upon transplantation in the liver. In response to injury, VESCs expand and regenerate entire vasculature structures, supporting the existence of an endothelial hierarchy within blood vessels. Genetic lineage tracing revealed that VESCs maintain large vessels and sinusoids in the normal liver for more than a year, and transplantation of VESCs rescued bleeding phenotypes in a mouse model of hemophilia. Our findings show that tissue-resident VESCs display self-renewal capacity and that vascular regeneration potential exists in peripheral blood vessels.

中文翻译：

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CD157标记具有稳态和再生特性的组织驻留内皮干细胞。

通过血管生成产生新血管对于满足组织氧需求至关重要。成体干细胞在此过程中的作用尚不清楚。在这里，我们鉴定出CD157（bst1，骨髓基质抗原1）是许多小鼠器官的大动脉和静脉中组织驻留的血管内皮干细胞（VESCs）的标记。单张CD157 ⁺VESC在体外形成菌落，并在肝移植后产生供体来源的门静脉，正弦波和中心静脉内皮细胞。作为对损伤的响应，VES​​C扩展并再生整个脉管系统结构，从而支持血管内内皮层级的存在。遗传谱系追踪显示，VESC在正常肝脏中维持大血管和正弦曲线超过一年，而VESC的移植在血友病小鼠模型中挽救了出血表型。我们的发现表明，驻留在组织中的VESC显示出自我更新的能力，并且外周血管中存在血管再生的潜力。