The inactivation of an isolated sulfonamide antibiotic resistant bacteria (ARB) HLS-6 and reduction of intracellular sul1 and intI1 in its genome by UVC irradiation, PMS oxidation and UVC-activated peroxymonosulfate (UVC/PMS) treatments were investigated in this study. The UVC/PMS treatment was superior to the other two methods in the inactivation of ARB and reduction of qPCR-sul1 and qPCR-intI1. The HLS-6 ARB (10⁸ CFU/mL) could be effectively inactivated 5.3 log by UVC (100 μW/cm²)/PMS (1 mg/L), and the reduction rates of qPCR-sul1 and qPCR-intI1 by UVC (100 μW/cm²)/PMS (20 mg/L), reached up to 2.9 log and 3.4 log, respectively within 30 min. qPCR-intI1 reacted faster than qPCR-sul1 in all methods. Sulfate radical was responsible for the reduction of target genes, while hydroxyl radical had negligible effect on that. The dosage of PMS positively affected the reduction of both genes during UVC/PMS, while the initial concentration of ARB could negatively influence the reduction of target genes. The pH 5 of reaction solution was most beneficial to the reduction of ARGs. The reduction rates at pH 5 reached up to 3.1 log (sul1) and 3.3 log (intI1). The reduction of target genes was slightly facilitated in the initial 5 min and suppressed after 5 min with the co-existence of sulfamethoxazole. This study will provide a potential alternative method for controlling the antibiotic resistance in aquatic environment.

通过UVC照射，PMS氧化和UVC活化的过一硫酸盐（UVC / PMS）处理，研究了分离的磺酰胺抗生素抗性细菌（ARB）HLS-6的失活以及其基因组中细胞内sul1和intI1的减少。该UVC / PMS治疗优于在ARB和减少qPCR-的失活的其他两种方法sul1和qPCR- intI1。的HLS-6 ARB（10 ⁸  CFU / mL）的可通过UVC（100μW/厘米被有效灭活5.3日志²）/ PMS（1毫克/升），和qPCR-的减少率sul1和qPCR- intI1通过UVC （100微瓦/厘米²/ PMS（20 mg / L），在30分钟内分别达到2.9 log和3.4 log。qPCR- intI1反应速度比qPCR- sul1中的所有方法。硫酸根自由基负责目标基因的还原，而羟基自由基对其的影响可忽略不计。PMS的剂量对UVC / PMS期间两个基因的减少有积极影响，而ARB的初始浓度可能对目标基因的减少有负面影响。反应溶液的pH 5最有利于减少ARGs。pH 5时的还原速率达到3.1 log（sul1）和3.3 log（intI1））。在最初的5分钟内略微促进了靶基因的减少，并在5分钟后与磺胺甲恶唑共存受到抑制。这项研究将为控制水生环境中的抗生素耐药性提供一种潜在的替代方法。