麻醉大鼠晶须丘脑反应的内侧前额叶皮质调节。,Neuroscience

当前位置： X-MOL 学术 › Neuroscience › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

麻醉大鼠晶须丘脑反应的内侧前额叶皮质调节。
Neuroscience ( IF 2.9 ) Pub Date : 2019-02-28 , DOI: 10.1016/j.neuroscience.2019.01.059
Guillermo Escudero ₁ , Angel Nuñez ₁

Affiliation

内侧前额叶皮层（mPFC）已牵涉到新颖性检测和注意。我们研究了mPFC电刺激对聚氨酯麻醉大鼠腹侧后丘脑内侧丘脑核（VPM），丘脑后丘脑核（POm）和主要体感（S1）皮质中记录的晶须响应的影响。在VPM或POm丘脑核，S1皮质和Zona Incerta（ZI）中进行了场电势和单位记录。晶须偏转引起体感诱发电位。电流脉冲由针对mPFC的前缘（PL）或下缘（IL）区域的双极刺激电极传递。PL列车刺激（50 Hz，500 ms）促进了持续数分钟的VPM核中的晶须响应，并在POm核中产生了短暂的抑制作用。IL刺激诱导VPM和POm核中晶须响应的促进。促成因是皮质皮质的预测，这是因为利多卡因使S1皮质失活后皮质皮质的表达减少了，而NMDA谷氨酸能受体的激活则是由于APV的阻断而促成的。mPFC和晶须的配对刺激显示了短时间间隔（<100 ms）的抑制作用，这是由ZI抑制神经元介导的，因为PL刺激诱导了大多数ZI神经元（42％）的响应促进作用，而麝香酚注射到ZI核中则降低了抑制作用。这表明mPFC可能通过激活GABA能ZI神经元来抑制POm神经元。总之，mPFC可以通过激活S1和ZI神经元来控制通过丘脑的体感信息流。

"点击查看英文标题和摘要"

Medial Prefrontal Cortical Modulation of Whisker Thalamic Responses in Anesthetized Rats.

The medial prefrontal cortex (mPFC) has been implicated in novelty detection and attention. We studied the effect of mPFC electrical stimulation on whisker responses recorded in the ventroposterior medial thalamic nucleus (VPM), the posterior thalamic nucleus (POm) and the primary somatosensory (S1) cortex in urethane anesthetized rats. Field potentials and unit recordings were performed in the VPM or POm thalamic nuclei, in S1 cortex, and in the Zona Incerta (ZI). Somatosensory evoked potentials were elicited by whisker deflections. Current pulses were delivered by bipolar stimulating electrodes aimed at the prelimbic (PL) or infralimbic (IL) areas of mPFC. PL train stimulation (50 Hz, 500 ms) induced a facilitation of whisker responses in the VPM nucleus that lasted minutes and a short inhibition in the POm nucleus. IL stimulation induced a facilitation of whisker responses in both VPM and POm nuclei. Facilitation was due to corticofugal projections because it was reduced after S1 cortical inactivation with lidocaine, and by activation of NMDA glutamatergic receptors because it was blocked by APV. Paired stimulation of mPFC and whiskers revealed an inhibitory effect at short intervals (<100 ms), which was mediated by ZI inhibitory neurons since PL stimulation induced response facilitation in the majority of ZI neurons (42%) and muscimol injection into ZI nucleus reduced inhibitory effects, suggesting that the mPFC may inhibit the POm neurons by activation of GABAergic ZI neurons. In conclusion, the mPFC may control the flow of somatosensory information through the thalamus by activation of S1 and ZI neurons.

更新日期：2019-02-28

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南