AbstractGraphene oxide (GO) is often quantified via its UV absorption, typically at around 230 nm. This is convenient but the effect of the size of GO on the accuracy of this method has been ignored so far. The authors report that the molar absorbance of GO is size-dependent. Data are presented on the absorbance of small (hydrodynamic diameter 1 μm), medium sized (1.5 μm), and large (2.2 μm) GO particles at wavelengths of 210, 230 and 250 nm. In general, linear relationship and good regression fits are obtained, but with different slope depending on size even at the same wavelength. This implies that using the UV absorption-based calibration may cause significant errors in GO quantification. Ultimately, this leads to incorrect dosages and faulty conclusions. This may also explain a variety of inconsistent results obtained in previous biological applications of GO. Graphical abstractThe size of graphene oxide (GO) determines its UV absorption and the UV absorption-based calibration (GO-s, GO-m and GO-l represent the GO with small, medium and large size).

中文翻译：

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氧化石墨烯的紫外吸收取决于尺寸：可能的校准缺陷

摘要氧化石墨烯 (GO) 通常通过其紫外线吸收进行量化，通常在 230 nm 左右。这很方便，但到目前为止，GO 的大小对这种方法的准确性的影响一直被忽略。作者报告说，GO 的摩尔吸光度与尺寸有关。数据显示在波长为 210、230 和 250 nm 的小（流体动力学直径 1 μm）、中型（1.5 μm）和大（2.2 μm）GO 颗粒的吸光度上。一般来说，可以获得线性关系和良好的回归拟合，但即使在相同的波长下，根据大小也有不同的斜率。这意味着使用基于 UV 吸收的校准可能会导致 GO 量化出现重大错误。最终，这会导致不正确的剂量和错误的结论。这也可以解释之前在 GO 的生物学应用中获得的各种不一致的结果。图形摘要氧化石墨烯（GO）的大小决定了它的紫外吸收和基于紫外吸收的校准（GO-s、GO-m和GO-l分别代表GO具有小、中、大尺寸）。