miR160和miR165 / 166之间的相互作用控制拟南芥叶片发育和耐旱性。,Scientific Reports

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miR160和miR165 / 166之间的相互作用控制拟南芥叶片发育和耐旱性。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-02-26 , DOI: 10.1038/s41598-019-39397-7
Tianxiao Yang _{1,

2} , Yongyan Wang _{1,

2} , Sachin Teotia _{1,

2,

3} , Zhaohui Wang ₁ , Chaonan Shi ₁ , Huwei Sun ₁ , Yiyou Gu ₂ , Zhanhui Zhang ₁ , Guiliang Tang _{1,

2}

Affiliation

微小RNA（miRNA）是一类非编码RNA，在植物发育和非生物胁迫中起重要作用。迄今为止，研究主要集中在单个miRNA的作用上，然而，少数研究解决了多个miRNA之间的相互作用。在这项研究中，我们使用短串联靶模拟物（STTM）研究了miR160和miR165 / 166之间的相互作用和调控回路及其对拟南芥叶片发育和干旱耐受性的影响。通过将STTM160拟南芥与STTM165 / 166杂交，我们成功地产生了miR160和miR165 / 166的双突变体。与单个STTM系中的表型改变相比，双突变体植物在叶片发育和干旱耐受性方面表现出一系列受损的表型。RNA-seq和qRT-PCR分析表明，与两个单一突变体相比，STTM定向双重突变体中生长素和ABA信号转导基因的表达水平受到了损害。我们的研究结果还表明，miR160指导的植物生长素应答因子（ARF）调节通过植物生长素信号传导基因促进叶片发育，而miR165 / 166介导的HD-ZIP IIIs调节则通过ABA信号传递赋予抗旱性。我们的研究进一步表明，ARF和HD-ZIP IIIs在调节叶片发育和抗旱性中可能发挥相反的作用，这些作用可进一步应用于其他农作物以改善农艺性状。我们的研究结果还表明，miR160指导的植物生长素应答因子（ARF）调节通过植物生长素信号传导基因促进叶片发育，而miR165 / 166介导的HD-ZIP IIIs调节则通过ABA信号传递赋予抗旱性。我们的研究进一步表明，ARF和HD-ZIP IIIs在调节叶片发育和抗旱性中可能发挥相反的作用，这些作用可进一步应用于其他农作物以改善农艺性状。我们的研究结果还表明，miR160指导的植物生长素应答因子（ARF）调节通过植物生长素信号传导基因促进叶片发育，而miR165 / 166介导的HD-ZIP IIIs调节则通过ABA信号传递赋予抗旱性。我们的研究进一步表明，ARF和HD-ZIP IIIs在调节叶片发育和抗旱性中可能发挥相反的作用，这些作用可进一步应用于其他农作物以改善农艺性状。

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The interaction between miR160 and miR165/166 in the control of leaf development and drought tolerance in Arabidopsis.

MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development and abiotic stresses. To date, studies have mainly focused on the roles of individual miRNAs, however, a few have addressed the interactions among multiple miRNAs. In this study, we investigated the interplay and regulatory circuit between miR160 and miR165/166 and its effect on leaf development and drought tolerance in Arabidopsis using Short Tandem Target Mimic (STTM). By crossing STTM160 Arabidopsis with STTM165/166, we successfully generated a double mutant of miR160 and miR165/166. The double mutant plants exhibited a series of compromised phenotypes in leaf development and drought tolerance in comparison to phenotypic alterations in the single STTM lines. RNA-seq and qRT-PCR analyses suggested that the expression levels of auxin and ABA signaling genes in the STTM-directed double mutant were compromised compared to the two single mutants. Our results also suggested that miR160-directed regulation of auxin response factors (ARFs) contribute to leaf development via auxin signaling genes, whereas miR165/166- mediated HD-ZIP IIIs regulation confers drought tolerance through ABA signaling. Our studies further indicated that ARFs and HD-ZIP IIIs may play opposite roles in the regulation of leaf development and drought tolerance that can be further applied to other crops for agronomic traits improvement.

更新日期：2019-02-26

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