A Pseudomonas sp. DNE-S1 (GenBank accession number MF803832), able to degrade DEP in a wide range of acid-base conditions, was isolated from landfill soil. The growth kinetics of DNE-S1 on DEP followed the inhibition model. Fe³⁺ could promote the degradation ability of DNE-S1 to DEP probably by over-expression of the gene phthalate dihydrodiol dehydrogenase (ophB) and phthalate dioxygenase ferredoxin reductase (ophA4). The degradation rate of DEP (500 mg L⁻¹ at 12 h) increased by 14.5% in the presence of Fe³⁺. Cu²⁺, Zn²⁺, and Mn²⁺ showed an inhibiting effect on the degradation performance of the strain and could alter the cellular morphology, surface area and volume of DNE-S1. Three degradation intermediates, namely ethyl methyl phthalate (EMP), dimethyl phthalate (DMP), and phthalic acid (PA), were detected in the biodegradation of DEP, and the biochemical pathway of DEP degradation was proposed. This study provides new information on the biochemical pathways and the responsible genes involved in DEP degradation.

一个假单胞菌属。从垃圾填埋场土壤中分离出了能够在广泛的酸碱条件下降解DEP的DNE-S1（GenBank登录号MF803832）。DNE-S1在DEP上的生长动力学遵循抑制模型。Fe ³⁺可能通过过量表达邻苯二甲酸二氢二醇脱氢酶（ophB）和邻苯二甲酸二加氧酶铁氧还蛋白还原酶（ophA4）来促进DNE-S1对DEP的降解能力。在存在Fe ³⁺的情况下，DEP的降解速率（12 h时为500 mg L ^-1）增加了14.5％。Cu 2 ⁺，Zn ²⁺和Mn ²⁺结果表明，该菌株对菌株的降解性能具有抑制作用，并且可以改变DNE-S1的细胞形态，表面积和体积。在DEP的生物降解过程中，检测到了三种降解中间体邻苯二甲酸乙酯（EMP），邻苯二甲酸二甲酯（DMP）和邻苯二甲酸（PA），并提出了DEP降解的生化途径。这项研究提供了有关DEP降解的生化途径和负责任基因的新信息。