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Vanilla bahiana, a contribution from the Atlantic Forest biodiversity for the production of vanilla: A proteomic approach through high-definition nanoLC/MS.
Food Research International ( IF 7.0 ) Pub Date : 2019-02-20 , DOI: 10.1016/j.foodres.2019.02.038 Ellen Moura Lopes 1 , Roberta Gomes Linhares 1 , Lucas de Oliveira Pires 2 , Rosane Nora Castro 2 , Gustavo Henrique Martins Ferreira Souza 3 , Maria Gabriela Bello Koblitz 4 , Luiz Claudio Cameron 5 , Andrea Furtado Macedo 6
Food Research International ( IF 7.0 ) Pub Date : 2019-02-20 , DOI: 10.1016/j.foodres.2019.02.038 Ellen Moura Lopes 1 , Roberta Gomes Linhares 1 , Lucas de Oliveira Pires 2 , Rosane Nora Castro 2 , Gustavo Henrique Martins Ferreira Souza 3 , Maria Gabriela Bello Koblitz 4 , Luiz Claudio Cameron 5 , Andrea Furtado Macedo 6
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Only a few cultivated species of Vanilla are used to produce vanilla, despite the high demand, predatory exploitation, and low genetic variability that threaten the production of natural vanilla. Vanilla bahiana pods from the Atlantic Forest may be an alternative source of natural vanilla. This study applied bottom-up and shotgun proteomics analysis to identify proteins related to flowering, fruiting, and vanilla-flavor production. Extraction solutions, including Tris-HCl buffer, β-mercaptoethanol and SDS, were assayed. SDS proved to be feasible for extraction of Vanilla fruit proteins and could be an alternative to the phenol method of protein extraction. Progenesis QI for Proteomics (QIP) software loaded with an Orchidaceae database identified 2326 proteins in our samples. Among these, 75 were highlighted as useful for the synthesis of compounds related to vanilla flavor, such as vanillin synthase, which was successfully extracted with 1% SDS, which also improved the variety of the extracted proteins. The proteins identified in V. bahiana pods indicate the enzymatic potential of this species, as further validated by quantifying the vanilla in the samples.
中文翻译：
Vanilla bahiana，来自大西洋森林生物多样性对香草生产的贡献：通过高清nanoLC / MS的蛋白质组学方法。
尽管需求旺盛，掠夺性开采和低遗传变异性威胁着天然香草的生产，但只有少数几种栽培香草用于生产香草。来自大西洋森林的香草巴哈那豆荚可能是天然香草的替代来源。这项研究应用了自下而上和shot弹枪蛋白质组学分析，以鉴定与开花，结果和香草味生产相关的蛋白质。分析了提取溶液，包括Tris-HCl缓冲液，β-巯基乙醇和SDS。SDS被证明是可行的，可用于提取香草果实蛋白，并且可以替代酚提取蛋白的方法。蛋白质组学的质子生成QI（QIP）软件装有兰花科数据库，可在我们的样品中鉴定出2326种蛋白质。在这些当中，其中75种被强调可用于合成与香草味有关的化合物，例如香兰素合酶，该香兰素合酶已成功用1％SDS提取，这也改善了提取蛋白质的种类。在巴氏烟草荚中鉴定出的蛋白质表明了该物种的酶促潜力，这通过对样品中的香草进行定量来进一步证实。
更新日期：2019-02-20
中文翻译：
Vanilla bahiana，来自大西洋森林生物多样性对香草生产的贡献：通过高清nanoLC / MS的蛋白质组学方法。
尽管需求旺盛，掠夺性开采和低遗传变异性威胁着天然香草的生产，但只有少数几种栽培香草用于生产香草。来自大西洋森林的香草巴哈那豆荚可能是天然香草的替代来源。这项研究应用了自下而上和shot弹枪蛋白质组学分析，以鉴定与开花，结果和香草味生产相关的蛋白质。分析了提取溶液，包括Tris-HCl缓冲液，β-巯基乙醇和SDS。SDS被证明是可行的，可用于提取香草果实蛋白，并且可以替代酚提取蛋白的方法。蛋白质组学的质子生成QI（QIP）软件装有兰花科数据库，可在我们的样品中鉴定出2326种蛋白质。在这些当中，其中75种被强调可用于合成与香草味有关的化合物，例如香兰素合酶，该香兰素合酶已成功用1％SDS提取，这也改善了提取蛋白质的种类。在巴氏烟草荚中鉴定出的蛋白质表明了该物种的酶促潜力，这通过对样品中的香草进行定量来进一步证实。
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