Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri‐arm star copolymers for gene delivery,Journal of Polymer Science Part A: Polymer Chemistry

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Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri‐arm star copolymers for gene delivery
Journal of Polymer Science Part A: Polymer Chemistry Pub Date : 2017-12-26 , DOI: 10.1002/pola.28938
Tianyuan Wu ₁ , Yu Cai ₁ , Xia Zhao ₂ , Chai K. Ngai ₁ , Benjamin Chu ₁ , Benjamin Hsiao ₁ , Michael Hadjiargyrou ₃ , Robert B. Grubbs ₁

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Amphiphilic cationic poly(ethylene oxide)‐S(polylysine)‐poly(d,l‐lactide) (mPEO‐S(CK_n)‐PLA) tri‐arm star copolymers were synthesized by a combination of ring opening polymerization (ROP) and a thiol–disulfide exchange. The mPEO‐S(CK_n)‐PLA copolymers were found to be non‐cytotoxic and could effectively condense GFP plasmid DNA into nanometer‐sized complexes, as characterized by dynamic light scattering (DLS), suitable for endocytotic cellular uptake. In vitro DNA transfection studies showed that the amphiphilic structure is capable of DNA transfection and GFP expression. Addition of chloroquine into the medium further enhanced the DNA transfection efficiency. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2018, 56, 635–644

中文翻译：

聚环氧乙烷/聚丙交酯/聚赖氨酸三臂星形共聚物的合成与表征

两性阳离子聚（环氧乙烷）-S（聚赖氨酸）-聚（d，l-丙交酯）（mPEO-S（CK _n）-PLA）三臂星形共聚物是通过开环聚合（ROP）和硫醇二硫化物交换。发现mPEO-S（CK _n）-PLA共聚物无细胞毒性，可以有效地将GFP质粒DNA浓缩成纳米大小的复合物，其特征在于动态光散射（DLS），适用于吞噬细胞。体外DNA转染研究表明，两亲结构能够进行DNA转染和GFP表达。向培养基中添加氯喹可进一步提高DNA转染效率。©2017 Wiley Periodicals，Inc. J. Polym。科学，A部分：Polym。化学 2018，56，635-644

更新日期：2017-12-26

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