内源性大麻素系统的成员在炎症性肠病和大肠癌中受到明显调节。,Scientific Reports

当前位置： X-MOL 学术 › Sci. Rep. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

内源性大麻素系统的成员在炎症性肠病和大肠癌中受到明显调节。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-02-20 , DOI: 10.1038/s41598-019-38865-4
Magdalena Grill ₁ , Christoph Högenauer _{2,

3} , Andreas Blesl ₂ , Johannes Haybaeck _{4,

5,

6} , Nicole Golob-Schwarzl ₅ , Nerea Ferreirós ₇ , Dominique Thomas ₇ , Robert Gurke ₇ , Martin Trötzmüller ₈ , Harald C Köfeler _{8,

9} , Birgit Gallé ₁₀ , Rudolf Schicho _{1,

3}

Affiliation

临床前研究表明，内源性大麻素系统（ECS）在预防肠道炎症和结直肠癌（CRC）方面起着重要作用。但是，人类数据很少。我们确定了炎症性肠病（IBD）和CRC患者的ECS成员和“内源性大麻素组”的相关组成部分，并将其与对照对象进行了比较。溃疡性结肠炎（UC）和克罗恩病（CD）患者的血浆中Anandamide（AEA）和油酰乙醇酰胺（OEA）升高，而CD患者中2-arachidonoylglycerol（2-AG）升高，而UC患者则不升高。CRC患者的2-AG升高，但AEA，PEA和OEA升高。IBD患者的溶血磷脂酰肌醇（LPI）18：0的水平高于对照组，而CRC和IBD的LPI 20：4均升高。肠粘膜活检中的基因表达揭示了CD和UC中的不同特征。CD，但不是UC患者，显示2-AG合成酶二酰基甘油脂肪酶α的基因表达增加。的成绩单CNR1和GPR119在CD中主要是减少。我们的数据显示，IBD和CRC中内源性大麻素和类内源性大麻素脂质的血浆水平发生了变化，而UC和CD中的转录本特征却有所不同。我们还报告了肠道炎症中鲜为人知的成分（例如GPR119，OEA和LPI）的改变。

"点击查看英文标题和摘要"

Members of the endocannabinoid system are distinctly regulated in inflammatory bowel disease and colorectal cancer.

Preclinical studies have demonstrated that the endocannabinoid system (ECS) plays an important role in the protection against intestinal inflammation and colorectal cancer (CRC); however, human data are scarce. We determined members of the ECS and related components of the ‘endocannabinoidome’ in patients with inflammatory bowel disease (IBD) and CRC, and compared them to control subjects. Anandamide (AEA) and oleoylethanolamide (OEA) were increased in plasma of ulcerative colitis (UC) and Crohn’s disease (CD) patients while 2-arachidonoylglycerol (2-AG) was elevated in patients with CD, but not UC. 2-AG, but not AEA, PEA and OEA, was elevated in CRC patients. Lysophosphatidylinositol (LPI) 18:0 showed higher levels in patients with IBD than in control subjects whereas LPI 20:4 was elevated in both CRC and IBD. Gene expression in intestinal mucosal biopsies revealed different profiles in CD and UC. CD, but not UC patients, showed increased gene expression for the 2-AG synthesizing enzyme diacylglycerol lipase alpha. Transcripts of CNR1 and GPR119 were predominantly decreased in CD. Our data show altered plasma levels of endocannabinoids and endocannabinoid-like lipids in IBD and CRC and distinct transcript profiles in UC and CD. We also report alterations for less known components in intestinal inflammation, such as GPR119, OEA and LPI.

更新日期：2019-02-20

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南