Homologous Sodium Alginate/Chitosan-Based Scaffolds, but Contrasting Effect on Stem Cell Shape and Osteogenesis,ACS Applied Materials & Interfaces

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Homologous Sodium Alginate/Chitosan-Based Scaffolds, but Contrasting Effect on Stem Cell Shape and Osteogenesis
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-02-19 00:00:00 , DOI: 10.1021/acsami.7b18859
Lili Zhang ₁ , Haowei Fang ₁ , Kunxi Zhang ₁ , Jingbo Yin ₁

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Stem cell shape appears to be involved in the regulation of osteogenesis, which has been confirmed in two-dimensional surfaces and three-dimensional hydrogels. The present study evaluated the effect of matrix-controlled cellular shape on osteogenesis in three-dimensional porous scaffolds based on sodium alginate (ALG) and chitosan (CS). Three ALG/CS scaffolds, especially including a stiff one, were fabricated from different precursor matrices. Soft scaffold A was fabricated from the ALG/CS polyelectrolyte and further cross-linked by Ca²⁺ and glutaraldehyde to achieve soft scaffold B with alternative hydrophilicity. Stiff scaffold C with “hard-to-deform” feature was fabricated from “ALG/CS preformed gel”, which was an ALG gel network expanded by swelling force of the dissolving CS, and fixed using Ca²⁺ and glutaraldehyde. Scanning electron microscopy and F-actins staining showed rounded mesenchymal stem cells (MSCs) on the inner surfaces inside scaffold A with high swelling behavior, but spindlelike MSCs in scaffold B. Stiff scaffold C forced MSCs to adhere to polygonal shape. Fibronectin adsorption was found to be weakened in scaffold A. Integrin α5β1 expression, as well as osteogenesis-related genes (ALP, OCN) expression, was detected to be higher in the stiff scaffold C. Thus, the present study illustrated that the stiff scaffold C responded to cells with hard-to-deform information, leading to the amplification of focal adhesions and induction of high tension of cells, consequently enhancement of osteogenesis.

中文翻译：

同源海藻酸钠/壳聚糖支架，但对干细胞形状和成骨作用相反

在二维表面和三维水凝胶中已证实，干细胞的形状似乎与成骨的调节有关。本研究评估了基于藻酸钠（ALG）和壳聚糖（CS）的三维多孔支架中基质控制的细胞形状对成骨作用的影响。用不同的前体基质制造了三个ALG / CS支架，特别是包括一个刚性支架。由ALG / CS聚电解质制备软支架A，并进一步通过Ca ²⁺和戊二醛交联，获得具有替代亲水性的软支架B。由“ ALG / CS预制凝胶”制成具有“难变形”特征的硬质支架C，这是一种通过溶解CS的溶胀力扩展并通过Ca固定的ALG凝胶网络。²⁺和戊二醛。扫描电子显微镜和F-肌动蛋白染色显示支架A内表面上的圆形间充质干细胞（MSC）具有高溶胀行为，但支架B中呈纺锤状MSC。刚性支架C迫使MSC粘附为多边形。发现在支架A中纤连蛋白的吸附减弱。在刚性支架C中检测到整合素α5β1表达以及成骨相关基因（ALP，OCN）的表达较高。因此，本研究表明，刚性支架C C对细胞具有难以变形的信息作出反应，从而导致粘着斑的放大和细胞高张力的诱导，因此增强了成骨作用。

更新日期：2018-02-19

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