Phenylpropanoids comprise an important class of plant secondary metabolites. A number of transcription factors have been used to upregulate-specific branches of phenylpropanoid metabolism, but by far the most effective has been the fruit-specific expression of AtMYB12 in tomato, which resulted in as much as 10% of fruit dry weight accumulating as flavonols and hydroxycinnamates. We show that AtMYB12 not only increases the demand of flavonoid biosynthesis but also increases the supply of carbon from primary metabolism, energy and reducing power, which may fuel the shikimate and phenylalanine biosynthetic pathways to supply more aromatic amino acids for secondary metabolism. AtMYB12 directly binds promoters of genes encoding enzymes of primary metabolism. The enhanced supply of precursors, energy and reducing power achieved by AtMYB12 expression can be harnessed to engineer high levels of novel phenylpropanoids in tomato fruit, offering an effective production system for bioactives and other high value ingredients.

中文翻译：

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多级工程促进了番茄中苯丙烷类化合物的生产。

苯丙烷类化合物是一类重要的植物次生代谢产物。许多转录因子已被用于上调苯丙氨酸代谢的特定分支，但到目前为止，最有效的是番茄中AtMYB12的果实特异性表达，这导致黄酮醇积累了多达10％的果实干重和羟基肉桂酸酯。我们表明，AtMYB12不仅增加了类黄酮生物合成的需求，而且还增加了来自初级代谢，能量和降低功率的碳供应，这可能会增加sh草酸酯和苯丙氨酸的生物合成途径，从而为次级代谢提供更多的芳香族氨基酸。AtMYB12直接结合编码初级代谢酶的基因的启动子。前体供应增加，