Separating and Profiling Phosphatidylcholines and Triglycerides from Single Cellular Lipid Droplet by In-Tip Solvent Microextraction Mass Spectrometry.,Analytical Chemistry

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Separating and Profiling Phosphatidylcholines and Triglycerides from Single Cellular Lipid Droplet by In-Tip Solvent Microextraction Mass Spectrometry.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-02-18 00:00:00 , DOI: 10.1021/acs.analchem.8b05122
Yaoyao Zhao ₁ , Zhen Chen ₁ , Yue Wu ₁ , Takayuki Tsukui ₂ , Xiaoxiao Ma ₃ , Xinrong Zhang ₄ , Hitoshi Chiba ₂ , Shu-Ping Hui ₁

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The analysis of lipid droplets (LDs) by mass spectrometry at the single LD level is still an analytical challenge. In this work, we developed a novel technique termed in-tip solvent microextraction mass spectrometry for the separation and profiling of phosphatidylcholines and triglycerides within a single LD. This method has been successfully used to analyze LDs in mammalian cells and to compare the profiles of triglycerides and phosphatidylcholines in LDs induced at different conditions. Our method has the potential to be applied to such fields as fundamental lipid biology to further our understanding on the mechanisms of lipid production, lipid packaging, and their pathophysiological roles.

中文翻译：

通过提示溶剂微萃取质谱法从单细胞脂质液滴中分离和分析磷脂酰胆碱和甘油三酸酯。

在单个LD水平上通过质谱分析脂滴（LD）仍然是一项分析挑战。在这项工作中，我们开发了一种称为尖端溶剂微萃取质谱的新技术，用于分离和分析单个LD中的磷脂酰胆碱和甘油三酸酯。该方法已成功用于分析哺乳动物细胞中的LDs，并比较了在不同条件下诱导的LDs中甘油三酸酯和磷脂酰胆碱的分布。我们的方法有潜力应用于基础脂质生物学等领域，以加深我们对脂质产生，脂质包装及其病理生理作用的理解。

更新日期：2019-02-18

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