In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions. The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme.

在这项工作中，已经研究了分子交联剂尺寸对金纳米粒子（AuNP）单层/多层结构特性，密度和均质性的影响。这些特征对于生物分子的功能结合非常重要。带正电荷的高或低分子量互连剂，聚L-赖氨酸（PLL）或N-（6-巯基）己基吡啶鎓（MHP）用于将带负电荷的AuNPs连接到平面金表面上并进一步互连通过一个多层结构逐层沉积。通过AuNPs分散液中的离子强度来调节组装好的AuNP膜中的颗粒间相互作用。通过带耗散的石英晶体微天平（QCM-D）技术评估了AuNP层的密度和结构/粘弹性。当比较组装的AuNP薄膜和牛上颌下腺黏液的均质层时，通过独立于模型的分析评估了专门为均质粘弹性薄膜的定量QCM-D数据分析开发的商业Voigt模型的有效性。AuNP单层和多层都可通过以下方式连接/互连与通过短MHP化合物相互连接的分子相比，较长的柔性PLL分子可组装成更致密，更柔软的粘稠结构。因此，通过定性评估酶的生物电化学特性，进一步研究了PLL互连的AuNP单/多层结构作为漆酶功能吸附的平台。