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Hydroxypropylcellulose Coating to Improve Graft-to-Bone Healing for Anterior Cruciate Ligament Reconstruction
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-02-18 00:00:00 , DOI: 10.1021/acsbiomaterials.8b01145 Jianjun Yang 1 , Yu Dong 2 , Jianguang Wang 1 , Chen Chen 3 , Yuchang Zhu 1 , Yang Wu 2 , Peng Zhang 2 , Tianwu Chen 2 , Weifeng Zhou 4 , Peiyi Wu 5 , Nguyen T. K. Thanh 6 , Ngoc Quyên Trân 7, 8 , Jun Chen 2 , Shiyi Chen 2
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-02-18 00:00:00 , DOI: 10.1021/acsbiomaterials.8b01145 Jianjun Yang 1 , Yu Dong 2 , Jianguang Wang 1 , Chen Chen 3 , Yuchang Zhu 1 , Yang Wu 2 , Peng Zhang 2 , Tianwu Chen 2 , Weifeng Zhou 4 , Peiyi Wu 5 , Nguyen T. K. Thanh 6 , Ngoc Quyên Trân 7, 8 , Jun Chen 2 , Shiyi Chen 2
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An anterior cruciate ligament (ACL) injury is one of the most common injuries in sports, and ACL reconstruction with an artificial ligament is a good treatment for quick recovery. However, current artificial ligaments made of polyethylene terephthalate (PET) are still associated with some problems due to the hydrophobic nature and low biological induction activity of PET. Many efforts have been used to improve the biocompatibility of PET in recent years, and our previous work has shown that surface modification is an effective strategy. Here, a hydroxypropylcellulose (HPC) coating was applied on the surface of a PET artificial ligament order to improve its biocompatibility. The effects of the HPC coating on PET artificial ligament graft-bone healing was investigated in vitro using bone marrow stromal cells (BMSCs), fibroblasts, and RSC-364 cells as well as in vivo in a beagle dog model of ACL reconstruction. HPC was coated successfully on the PET and significantly promoted cell growth, adhesion, and capability of osteogenic differentiation compared to the PET graft without HPC coating. In vivo, the HPC coating significantly enhanced ligament tissue regeneration. Moreover, higher expression of some bone-formation- and ligament-tissue-regeneration-contributing proteins and cell factors, such as COL1, BMP-7, COL3, OCN, RUNX2, TGF-β1, and VEGF, was observed on the HPC-coated PET artificial ligament in comparison with the pure PET artificial ligament. In conclusion, HPC coating can significantly improve the cytocompatibility and graft-to-bone healing of a PET artificial ligament for ACL reconstruction.
中文翻译：
羟丙基纤维素涂层可改善前交叉韧带重建的植骨愈合。
前十字韧带（ACL）损伤是运动中最常见的损伤之一，而用人工韧带重建ACL是快速恢复的好方法。然而，由于PET的疏水性和低的生物诱导活性，目前由聚对苯二甲酸乙二酯（PET）制成的人造韧带仍然存在一些问题。近年来，已经进行了许多努力来改善PET的生物相容性，并且我们以前的工作表明表面改性是一种有效的策略。此处，将羟丙基纤维素（HPC）涂层涂覆在PET人造韧带的表面上，以改善其生物相容性。体外研究了HPC涂层对PET人造韧带移植骨愈合的影响在ACL重建的比格犬模型中，使用骨髓基质细胞（BMSC），成纤维细胞和RSC-364细胞以及体内实验。与没有HPC涂层的PET移植物相比，HPC成功地涂覆在PET上，并显着促进了细胞生长，粘附和成骨分化能力。体内，HPC涂层显着增强了韧带组织的再生。此外，在HPC-上观察到一些骨形成和韧带组织再生的蛋白质和细胞因子（例如COL1，BMP-7，COL3，OCN，RUNX2，TGF-β1和VEGF）的更高表达。与纯PET人造韧带相比，涂层PET人造韧带。总之，HPC涂层可以显着改善用于ACL重建的PET人造韧带的细胞相容性和移植物至骨的愈合。
更新日期：2019-02-18
中文翻译：
羟丙基纤维素涂层可改善前交叉韧带重建的植骨愈合。
前十字韧带（ACL）损伤是运动中最常见的损伤之一，而用人工韧带重建ACL是快速恢复的好方法。然而，由于PET的疏水性和低的生物诱导活性，目前由聚对苯二甲酸乙二酯（PET）制成的人造韧带仍然存在一些问题。近年来，已经进行了许多努力来改善PET的生物相容性，并且我们以前的工作表明表面改性是一种有效的策略。此处，将羟丙基纤维素（HPC）涂层涂覆在PET人造韧带的表面上，以改善其生物相容性。体外研究了HPC涂层对PET人造韧带移植骨愈合的影响在ACL重建的比格犬模型中，使用骨髓基质细胞（BMSC），成纤维细胞和RSC-364细胞以及体内实验。与没有HPC涂层的PET移植物相比，HPC成功地涂覆在PET上，并显着促进了细胞生长，粘附和成骨分化能力。体内，HPC涂层显着增强了韧带组织的再生。此外，在HPC-上观察到一些骨形成和韧带组织再生的蛋白质和细胞因子（例如COL1，BMP-7，COL3，OCN，RUNX2，TGF-β1和VEGF）的更高表达。与纯PET人造韧带相比，涂层PET人造韧带。总之，HPC涂层可以显着改善用于ACL重建的PET人造韧带的细胞相容性和移植物至骨的愈合。
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