The objective of this study was to develop a consecutive preparation method for the isolation and purification of hainanmurpanin, meranzin, and phebalosin from leaves of Murraya exotica L. The process involved supercritical fluid extraction with CO₂, solvent extraction, and two‐step high‐speed countercurrent chromatography. Pressure, temperature, and the volume of entrainer were optimized as 27 MPa, 52°C, and 60 mL by response surface methodology in supercritical fluid extraction with CO₂, and the yield of the crude extracts was 7.91 g from 100 g of leaves. Subsequently, 80% methanol/water was used to extract and condense the three compounds from the crude extracts, and 4.23 g of methanol/water extracts was obtained. Then, a two‐step high‐speed countercurrent chromatography procedure was developed for the isolation of the three target compounds from methanol/water extracts, including conventional high‐speed countercurrent chromatography for further enrichment and consecutive high‐speed countercurrent chromatography for purification. The yield of concentrates from high‐speed countercurrent chromatography was 2.50 g from 4.23 g of methanol/water extracts. Finally, the consecutive high‐speed countercurrent chromatography produced 103.2 mg of hainanmurpanin, 244.7 mg of meranzin, and 255.4 mg of phebalosin with purities up to 97.66, 99.36, and 98.64%, respectively, from 900 mg of high‐speed countercurrent chromatography concentrates in one run of three consecutive sample loadings without exchanging a solvent system.

中文翻译：

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超临界流体萃取-连续高速逆流色谱法从穆拉亚叶中制备海南紫花pan素，黑色素和酞菁蛋白的分离纯化

本研究的目的是开发一种用于从叶分离和hainanmurpanin，meranzin的纯化和phebalosin连续制备方法九里香L.该过程涉及超临界流体萃取的CO ₂，溶剂萃取，和两步高快速逆流色谱。在CO ₂超临界流体萃取中，通过响应表面方法将压力，温度和夹带剂的体积优化为27 MPa，52°C和60 mL，从100克树叶中提取的粗提物的产量为7.91克。随后，使用80％的甲醇/水从粗提取物中提取和浓缩三种化合物，得到4.23g的甲醇/水提取物。然后，开发了一种两步高速逆流色谱方法，用于从甲醇/水提取物中分离出三种目标化合物，包括常规的高速逆流色谱以进一步富集，以及连续的高速逆流色谱以进行纯化。高速逆流色谱法从4.23 g甲醇/水提取物中得到的浓缩物产量为2.50 g。最后，连续的高速逆流色谱法产生的纯净度高达97.66，产生了103.2 mg hainanmurpanin，244.7 mg melanzin和255.4 mg phebalosin，