<br>YeeJ 是一种来自大肠杆菌的反向自转运蛋白，可与肽聚糖结合并促进生物膜形成。,Scientific Reports

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YeeJ 是一种来自大肠杆菌的反向自转运蛋白，可与肽聚糖结合并促进生物膜形成。
Scientific Reports ( IF 3.8 ) Pub Date : 2017-Sep-12 , DOI: 10.1038/s41598-017-10902-0
Marta Martinez-Gil _{1,

2} , Kelvin G K Goh _{3,

4} , Elze Rackaityte _{1,

5} , Chizuko Sakamoto ₁ , Bianca Audrain ₁ , Danilo G Moriel _{3,

4,

6} , Makrina Totsika _{3,

4,

7} , Jean-Marc Ghigo ₁ , Mark A Schembri _{3,

4} , Christophe Beloin ₁

Affiliation

大肠杆菌是一种可以在多种环境中生存的共生或致病细菌。表面粘附对于大肠杆菌定植至关重要，因此了解在不同生态位中促进这一过程的分子机制非常重要。自转运蛋白是大肠杆菌用于粘附的一类细胞表面因子。在这里，我们描述了 YeeJ 的调控和功能，YeeJ 是一类新兴的自转运蛋白（逆自转运蛋白（IAT））的研究很少但广泛的代表。我们发现 yeeJ 基因存在于 96 个完整测序的大肠杆菌基因组中的约 40% 中，并且 YeeJ 以两种长度变体的形式存在，尽管没有可检测到的功能差异。我们证明 YeeJ 通过暴露在细胞表面促进不同环境下生物膜的形成。我们还表明 YeeJ 包含一个与肽聚糖相互作用的 LysM 结构域，从而有助于其定位到外膜中。此外，我们还鉴定出多核苷酸磷酸化酶 PNPase 作为 yeeJ 转录的抑制因子。总的来说，我们的工作为 YeeJ 作为最近定义的 IAT 类的成员提供了新的见解，并有助于我们了解共生和致病性大肠杆菌如何在其环境中定殖。

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YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation.

Escherichia coli is a commensal or pathogenic bacterium that can survive in diverse environments. Adhesion to surfaces is essential for E. coli colonization, and thus it is important to understand the molecular mechanisms that promote this process in different niches. Autotransporter proteins are a class of cell-surface factor used by E. coli for adherence. Here we characterized the regulation and function of YeeJ, a poorly studied but widespread representative from an emerging class of autotransporter proteins, the inverse autotransporters (IAT). We showed that the yeeJ gene is present in ~40% of 96 completely sequenced E. coli genomes and that YeeJ exists as two length variants, albeit with no detectable functional differences. We demonstrated that YeeJ promotes biofilm formation in different settings through exposition at the cell-surface. We also showed that YeeJ contains a LysM domain that interacts with peptidoglycan and thus assists its localization into the outer membrane. Additionally, we identified the Polynucleotide Phosphorylase PNPase as a repressor of yeeJ transcription. Overall, our work provides new insight into YeeJ as a member of the recently defined IAT class, and contributes to our understanding of how commensal and pathogenic E. coli colonise their environments.

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